Anti-Mumps nucleoprotein 抗体 [5E3] (ab9879)

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Key features and details

  • Mouse monoclonal [5E3] to Mumps nucleoprotein
  • Suitable for: Indirect ELISA, WB
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-Mumps nucleoprotein antibody [5E3]
    Mumps nucleoprotein 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [5E3] to Mumps nucleoprotein

  • 由来種

    Mouse

  • アプリケーション

    適用あり: Indirect ELISA, WBmore details

  • 種交差性

    交差種: Other species

  • 免疫原

    Recombinant mumps NP. Gloucester strain (Glouc 1/UK96)

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.1% Sodium azide
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    5E3

  • ミエローマ

    NS0/1

  • アイソタイプ

    IgG2a

  • 軽鎖の種類

    unknown

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Indirect ELISA
WB
  • 追加情報
    Indirect ELISA: Use at 1/1000 to 1/5000
    Western Blot: Use at 1/500 to 1/1000

    • ターゲット情報

    画像

    • Western blot - Anti-Mumps nucleoprotein antibody [5E3] (ab9879)
      Western blot - Anti-Mumps nucleoprotein antibody [5E3] (ab9879)

      Western blot of yeast-derived mumps NP using ab9879 at a concentration of 0.5 µg/ml.

      Western blot of yeast-derived mumps NP using ab9879 at a concentration of 0.5 µg/ml.

    参考文献 (2)

    ab9879 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9879 は 2 報の論文で使用されています。

    • Samuel D  et al. High level expression of recombinant mumps nucleoprotein in Saccharomyces cerevisiae and its evaluation in mumps IgM serology. J Med Virol 66:123-30 (2002). PubMed: 11748668
    • A.Þvirblienë  et al. Studies on the structure and antigenic properties of mumps virus nucleocapsid protein produced in yeast cells. Immunol Lett. Abs. of 14th Eur Immunol. meet. EFIS : (2000). PubMed:

    レビューと Q&A

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    Question

    Please provide details of the stains of mumps virus that Abcam mumps antibodies have been tested on.

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    Answer

    Thank you for your recent telephone enquiry. I can confirm that ab9879,ab9880 and ab9392 have been created using nuclear protein from Recombinant mumps NP.Gloucester strain (Glouc 1/UK96). Antibodies ab9876 and ab9878 have been raised using recombiant NP protein. I am unable to find data on which strain this is taken from. I can confirm all of these antibodies (ab9392,ab9876, ab9878, ab9879, ab9880) have also been succesfully tested in mumps strains 55799-98 and SJ801-95. I can suggest checking the sequence alignment of the NP protein of the different strains you are interested in. We recommned that sequence homology should be over 85% to predict an antibody will detect a protein. Mumps antibody ab20859 has been tested sucessfully on the Enders strain (from which the immunogen was taken) and a CDC strain #V5-004. Other strains (including 55799-98 and SJ801-95 and Gloucester) have not been tested, including 55799-98 and SJ801-95 and Gloucester. Again, I can suggest conducting a sequence alignment search to see which strains may be detectable. I hope this information is helpful. Should you require any further assistance, please do not hesitate to contact us again.

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