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Anti-Mucin 5AC 抗体 [EPR16904] - BSA and Azide free (ab271960)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free (ab271960)
  • Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free (ab271960)
  • Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free (ab271960)
  • Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free (ab271960)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16904] to Mucin 5AC - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free
    Mucin 5AC 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR16904] to Mucin 5AC - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human stomach lysate. IHC-P: Human stomach tissue. ICC/IF: HeLa and SW480 cells.

  • 特記事項

    ab271960 is the carrier-free version of ab198294. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC/IF
Human
IHC-P
Human
アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 250-600 kDa (predicted molecular weight: 585 kDa).
特記事項
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 250-600 kDa (predicted molecular weight: 585 kDa).

ターゲット情報

  • 関連性

    Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen.

  • 細胞内局在

    Secreted; Cytoplasmic and cell surface

  • 参照データベース

    • 別名

      • Apomucin antibody
      • Apomucin Major Airway Glycoprotein antibody
      • Gastric mucin antibody
      • LeB antibody
      • Lewis B blood group antigen antibody
      • Major Airway Glycoprotein antibody
      • MUC 5 antibody
      • MUC5 antibody
      • MUC5AC antibody
      • Mucin 5 subtype AC tracheobronchial antibody
      • Mucin 5 Subtypes A And C antibody
      • Mucin 5 subtypes A and C tracheobronchial/gastric antibody
      • Mucin 5, tracheobronchial/gastric antibody
      • Mucin 5AC antibody
      • Mucin 5AC oligomeric mucus/gel forming antibody
      • Mucin 5AC, oligomeric mucus/gel forming pseudogene antibody
      • Mucin antibody
      • TBM antibody
      • Tracheobronchial Mucin (TBM) antibody
      • Tracheobronchial mucin antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free (ab271960)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free (ab271960)

      Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Mucin 5AC with ab198294 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Cytoplasm staining on Human stomach tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198294).
    • Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free (ab271960)
      Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free (ab271960)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Mucin 5AC with ab198294 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

      Cytoplasm staining on HeLa cell line is observed.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:-
      -ve control 1: ab198294 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198294).
    • Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free (ab271960)
      Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free (ab271960)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (Human colorectal adenocarcinoma cell line) cells labeling Mucin 5AC with ab198294 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

      Cytoplasm staining on SW480 cell line is observed.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:-
      -ve control 1: ab198294 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198294).
    • Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free (ab271960)
      Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free (ab271960)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab271960 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab271960 は論文での使用が確認できていません。

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