Anti-MUC5B 抗体 [6F10-E4] (ab105460)
Key features and details
- Mouse monoclonal [6F10-E4] to MUC5B
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-MUC5B antibody [6F10-E4]
MUC5B 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [6F10-E4] to MUC5B -
由来種
Mouse -
特異性
Non glycosylated domain of mucin MUC5B. -
アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human MUC5B conjugated to keyhole limpet haemocyanin. Synthetic peptide from 4 of the cysteine domains of the tandem repeat region.
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特記事項
Detection of respiratory and salivary mucins in clinical and experimental material.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
一次抗体 備考
Detection of respiratory and salivary mucins in clinical and experimental material. -
ポリ/モノ
モノクローナル -
クローン名
6F10-E4 -
ミエローマ
Sp2 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab105460の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 596 kDa. PubMed: 14678646
Please consult PubMed ID 14678646 for the recommended electrophoresis conditions. |
| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 596 kDa. PubMed: 14678646 Please consult PubMed ID 14678646 for the recommended electrophoresis conditions. |
ターゲット情報
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機能
Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus. -
組織特異性
Expressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas. -
配列類似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 3 HAT repeats.
Contains 3 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
ドメイン
The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding. -
翻訳後修飾
Highly glycosylated. C-, N- and O-glycosylated. C-mannosylated in the Cys-rich subdomains probably on the first Trp residue of the WXXW motif. Highly O-glycosylated in the Ser/Thr-rich tandem repeat (TR) region. The repeat region is about 59% O-glycosylated with a high abundance of NeuAc(2)Hex(1)HexNac1-ol. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 727897 Human
- Omim: 600770 Human
- SwissProt: Q9HC84 Human
- Unigene: 523395 Human
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別名
- 2300002I04Rik antibody
- A130042M24 antibody
- AV085033 antibody
see all
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (5)
ab105460 は 5 報の論文で使用されています。
- Saitou M et al. Functional Specialization of Human Salivary Glands and Origins of Proteins Intrinsic to Human Saliva. Cell Rep 33:108402 (2020). PubMed: 33207190
- Martinez-Valdebenito C et al. Characterization of Oral Immunity in Cases and Close Household Contacts Exposed to Andes Orthohantavirus (ANDV). Front Cell Infect Microbiol 10:557273 (2020). PubMed: 33224896
- van Leeuwen S et al. Early salivary changes in multiple myeloma patients undergoing autologous HSCT. Oral Dis 24:972-982 (2018). PubMed: 29637662
- Lachowicz-Scroggins ME et al. Abnormalities in MUC5AC and MUC5B Protein in Airway Mucus in Asthma. Am J Respir Crit Care Med 194:1296-1299 (2016). PubMed: 27845589
- Rousseau K et al. New monoclonal antibodies to non-glycosylated domains of the secreted mucins MUC5B and MUC7. Hybrid Hybridomics 22:293-9 (2003). WB, ELISA ; Human . PubMed: 14678646
