Application
Western blot
Sample
Rat Tissue lysate - whole (GI)
Loading amount
50 µg
Specification
GI
Gel Running Conditions
Reduced Denaturing
Blocking step
proprietary as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFluor700 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HiLyte Plus™ 647
Host species: Goat
Clonality: Polyclonal
Conjugation: HiLyte Plus™ 647
Detection
Detection method
fluorescent scanner
Exposure
5 minute(s) and 0 second(s)
Additional data
Abcam response
ab7848 has not been tested in WB or on Rat so it may or may not work in this application or species. This product is a mouse antibody so an anti-rabbit secondary antibody would not be compatible - this is probably the cause of the problem.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Sep 27 2007