Key features and details
- Mouse monoclonal [B306.1] to MUC2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
製品名Anti-MUC2 antibody [B306.1]
MUC2 一次抗体 製品一覧
製品の詳細Mouse monoclonal [B306.1] to MUC2
特異性This antibody reacts with MUC2 gene product, a secretory apomucin found in a variety of normal and neoplastic human glandular epithelia.
アプリケーション適用あり: IHC-P, WBmore details
Synthetic MUC2 tandem repeat consensus sequence.
- Colon carcinoma
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS
Concentration information loading...
精製度Protein G purified
Our Abpromise guarantee covers the use of ab7848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/4000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration.|
機能Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
組織特異性Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
配列類似性Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 TIL (trypsin inhibitory-like) domain.
Contains 2 VWFC domains.
Contains 4 VWFD domains.
May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
細胞内局在Secreted. In the intestine, secreted into the inner and outer mucus layers.
- Information by UniProt
- Intestinal mucin 2 antibody
- Intestinal mucin-2 antibody
- MLP antibody
Ab7848 staining human normal colon. Staining is localised to the membrane.
Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab7848 は 1 報の論文で使用されています。
- Fishbein TM et al. Rejection reversibly alters enteroendocrine cell renewal in the transplanted small intestine. Am J Transplant 9:1620-8 (2009). IHC-Fr ; Human . PubMed: 19519821