Application
ELISA
Sample
Human Purified protein (duodenal extracts)
Specification
duodenal extracts
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 37°C
Type
Indirect
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS-1%BSA
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: anti-mouse IgG (H+L)-HRP (Uptima)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Biotinylated Wheat Germ Agglutinin was used as a positive control in this test as it binds to glycosylation present in intestinal extracts.
Abcam response
Abcam has not tested ab11197 in ELISA and thus can't say how it should have performed. This individual received a credit for testing this antibody in ELISA as part of our AbTrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 12 2019