製品の概要

  • 製品名
    Anti-MUC16 antibody [X75]
    MUC16 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [X75] to MUC16
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    CA-125 purified from human ovarian carcinoma.

  • ポジティブ・コントロール
    • human adenocarcinoma or ovarian carcinoma cell lines or tissues
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibodies ab110640 or ab134093 as replacements.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 24603616

ターゲット情報

  • 機能
    Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
  • 組織特異性
    Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
  • 配列類似性
    Contains 2 ANK repeats.
    Contains 56 SEA domains.
  • ドメイン
    Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
  • 翻訳後修飾
    Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
    Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
    May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
    May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
  • 細胞内局在
    Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CA 125 antibody
    • CA-125 antibody
    • CA125 antibody
    • CA125 ovarian cancer antigen antibody
    • Cancer antigen 125 antibody
    • FLJ14303 antibody
    • MUC 16 antibody
    • MUC-16 antibody
    • MUC16 antibody
    • MUC16_HUMAN antibody
    • Mucin 16 antibody
    • Mucin 16 cell surface associated antibody
    • Mucin-16 antibody
    • Mucin16 antibody
    • Ovarian cancer related tumor marker CA125 antibody
    • Ovarian cancer-related tumor marker CA125 antibody
    • Ovarian carcinoma antigen CA125 antibody
    see all

画像

  • Overlay histogram showing HeLa cells stained with ab1107 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1107, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed HeLa cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • IHC image of ab1107 staining in human breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1107, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

This product has been referenced in:
  • Vitiazeva V  et al. The O-Linked Glycome and Blood Group Antigens ABO on Mucin-Type Glycoproteins in Mucinous and Serous Epithelial Ovarian Tumors. PLoS One 10:e0130197 (2015). Read more (PubMed: 26075384) »
  • Zhang H  et al. Relationship of tumor marker CA125 and ovarian tumor stem cells: preliminary identification. J Ovarian Res 8:19 (2015). Read more (PubMed: 25886959) »
See all 7 Publications for this product

レビューと Q&A

1-7 of 7 Abreviews or Q&A

Question
Answer

Thank you for contacting us.


The Isotype control for product ab1107 will be ab80201; https://www.abcam.com/Mouse-IgG1-CT6-Isotype-control-ab81216.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for contacting us. We offer many thousand products, it seems possible that in this particular instance we are offering the same monoclonal antibody from differing sources. Because the clone number is the same for both products, I am inclined to believe that they will have similar response in similar assays. However as the separate products have not been tested in the same assays we cannot guarantee that using one in an assay that the second has been tested for will produce acceptable results. We do have a program in place to for cases where we cannot guarantee the product only because it has not been tested in a particular assay, and where the item is know to react with the species type to be used or a species with >85% homology. This is our 100% Abreview discount. I can offer a discount off a future purchase if you buythe untested antibodynow, test it and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and testthis. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering. 2. Purchase either by phone, fax, or online (www.abcam.com). 3. Test it in the untested assay. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifit turns out to be unsuitable, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (Rat ovarian cancer)
Specification
Rat ovarian cancer
Preparation
Cell harvesting/tissue preparation method: Trypsinization for 4-8 minutes at 37 degrees C
Sample buffer: PBS
Fixation
none
Permeabilization
No
Gating Strategy
Living cells by FSC vs SSC

Abcam user community

Verified customer

投稿 Oct 03 2008

Answer

Hello, When the datasheets were created the clone was not added to any of them. Sorry. I don't know the reason why but none of our datasheets show the clone. Thanks, Amber Castle -----Original Message----- From: technical@abcam.com [mailto:technical@abcam.com] Sent: Monday, October 30, 2006 8:50 AM To: Amber@qedbio.com Subject: Abcam question regarding 3330 - [CID137780] Any insight would be much appreciated! Thanks, Bonnie -------------------- Customer enquiry: Hi Amber, This is strange indeed! We'd like to add it to our datasheet but is there a reason it's not on your datasheet? -------------------- Your contact reference is [CID137780] Please keep it in the subject line or body of the email when you reply.

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Answer

Thank you for your enquiry. The antibody ab1107 has been tested in paraffin-embedded tissues of human adenocarcinoma and ovarian carcinoma. We do not know if CA-125 is trypsin-sensitive unfortunately.

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Answer

Thank you for your enquiry. For ab11387, the half-life is not known. In addition I would like to refer you to the following publication for information on the molecular weight. It appears to be about 23kDa. The citation details are as follows: Berger P et al (1993) J. Clin. Endocrinol. Metab. 77(2):347-351. For ab1107, the molecular weight is approximately 150 kDa and the half-life is unknown. Regarding both, we don't have any information about whether they could go through a humanisation process.

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Question
Answer

Ab1107 is an IgG1 antibody, so the molecular weight of this will be in the range of 155-165 kDa.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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