Anti-MUC16 抗体 [OV185:1] (ab697)

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製品の概要

  • 製品名

    Anti-MUC16 antibody [OV185:1]
    MUC16 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [OV185:1] to MUC16

  • 由来種

    Mouse

  • アプリケーション

    適用あり: Flow Cyt, IHC-P, IHC-Frmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Partially purified human mucin fraction from a pool of cancer tissues of patients with epithelial ovarian.

  • ポジティブ･コントロール

    • Ovarian carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-P Use at an assay dependent concentration. 30 - 60 mins at RT (ABC method). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

  • 組織特異性

    Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.

  • 配列類似性

    Contains 2 ANK repeats.
    Contains 56 SEA domains.

  • ドメイン

    Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.

  • 翻訳後修飾

    Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
    Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
    May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
    May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.

  • 細胞内局在

    Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CA 125 antibody
      • CA-125 antibody
      • CA125 antibody
      • CA125 ovarian cancer antigen antibody
      • Cancer antigen 125 antibody
      • FLJ14303 antibody
      • MUC 16 antibody
      • MUC-16 antibody
      • MUC16 antibody
      • MUC16_HUMAN antibody
      • Mucin 16 antibody
      • Mucin 16 cell surface associated antibody
      • Mucin-16 antibody
      • Mucin16 antibody
      • Ovarian cancer related tumor marker CA125 antibody
      • Ovarian cancer-related tumor marker CA125 antibody
      • Ovarian carcinoma antigen CA125 antibody
      see all

    画像

    • Flow Cytometry - Anti-MUC16 antibody [OV185:1] (ab697)
      Flow Cytometry - Anti-MUC16 antibody [OV185:1] (ab697)
      Overlay histogram showing HeLa cells stained with ab697 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab697, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    参考文献

    This product has been referenced in:

    • Kobayashi Y  et al. Generation of a TALEN-mediated, p63 knock-in in human induced pluripotent stem cells. Stem Cell Res 25:256-265 (2017). IF ; Human . Read more (PubMed: 29179035) »
    See 1 Publication for this product

    ab697 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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