製品の概要
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製品名Anti-MUC16 antibody [OC125]
MUC16 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [OC125] to MUC16
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由来種Mouse
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特異性Studies have show that this antibody reacts with approximately 80% of epithelial ovarian neoplasms of serous, endometrioid, clear cell and undifferentiated types. No reactivity has been shown for mucinous ovarian tumors or in germ cell or hematopoietic tumors. It reacts with both normal tissues and neoplasms of fallopian tube, endometrium, endocervix and mesothelioma. It does not react with colon cancer. Normal tissues such as breast, liver, skin, kidney and spleen are negative.
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アプリケーション適用あり: Flow Cyt, IHC-Pmore details
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種交差性交差種: Human
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免疫原
Partially purified human mucin fraction from a pool of tissues from patients with epithelial ovarian cancer.
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エピトープThis antibody recognizes an epitope on a molecule called Cancer Antigen 125 (CA125).
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ポジティブ･コントロール
- Flow cytometry: HeLa cells. IHC-P: Ovarian cancer tissue.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.30
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA, Renoir Red diluent -
Concentration information loading... -
ポリ/モノモノクローナル
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クローン名OC125
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アイソタイプIgG1
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研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Unmasking reagent
アプリケーション
Our Abpromise guarantee covers the use of ab693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC Method. |
ターゲット情報
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機能Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
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組織特異性Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
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配列類似性Contains 2 ANK repeats.
Contains 56 SEA domains. -
ドメインComposed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
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翻訳後修飾Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. -
細胞内局在Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
- Information by UniProt
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参照データベース
- Entrez Gene: 94025 Human
- Omim: 606154 Human
- SwissProt: Q8WXI7 Human
- Unigene: 432676 Human
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別名
- CA 125 antibody
- CA-125 antibody
- CA125 antibody
see all
画像
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![Flow Cytometry - Anti-MUC16 antibody [OC125] (ab693)](https://www.abcam.co.jp/ps/products/0/ab693/Images/MUC16-Primary-antibodies-ab693-1.jpg)
Flow Cytometry - Anti-MUC16 antibody [OC125] (ab693)Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab693 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab693, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [OC125] (ab693)](https://www.abcam.co.jp/ps/products/0/ab693/Images/ab693-284421-ab6931333.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [OC125] (ab693)Paraffin embedded human ovarian cancer tissue stained for MUC16 with ab693 (1/50 dilution) in immunohistochemical analysis.
参考文献
This product has been referenced in:
- Kline JB et al. Tumor antigen CA125 suppresses antibody-dependent cellular cytotoxicity (ADCC) via direct antibody binding and suppressed Fc-? receptor engagement. Oncotarget 8:52045-52060 (2017). Flow Cyt ; Human . Read more (PubMed: 28881712) »
- Kim BR et al. SOX2 and PI3K Cooperate to Induce and Stabilize a Squamous-Committed Stem Cell Injury State during Lung Squamous Cell Carcinoma Pathogenesis. PLoS Biol 14:e1002581 (2016). ICC/IF ; Human . Read more (PubMed: 27880766) »
レビューと Q&A
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