Anti-MUC16 抗体 [OC125] (ab693)
Key features and details
- Mouse monoclonal [OC125] to MUC16
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-MUC16 antibody [OC125]
MUC16 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [OC125] to MUC16 -
由来種
Mouse -
特異性
Studies have show that this antibody reacts with approximately 80% of epithelial ovarian neoplasms of serous, endometrioid, clear cell and undifferentiated types. No reactivity has been shown for mucinous ovarian tumors or in germ cell or hematopoietic tumors. It reacts with both normal tissues and neoplasms of fallopian tube, endometrium, endocervix and mesothelioma. It does not react with colon cancer. Normal tissues such as breast, liver, skin, kidney and spleen are negative. -
アプリケーション
適用あり: Flow Cyt, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Partially purified human mucin fraction from a pool of tissues from patients with epithelial ovarian cancer.
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エピトープ
This antibody recognizes an epitope on a molecule called Cancer Antigen 125 (CA125). -
ポジティブ・コントロール
- Flow cytometry: HeLa cells. IHC-P: Ovarian cancer tissue.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA, Renoir Red diluent -
Concentration information loading...
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ポリ/モノ
モノクローナル -
クローン名
OC125 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC Method. |
ターゲット情報
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機能
Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. -
組織特異性
Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas. -
配列類似性
Contains 2 ANK repeats.
Contains 56 SEA domains. -
ドメイン
Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail. -
翻訳後修飾
Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. -
細胞内局在
Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain. - Information by UniProt
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参照データベース
- Entrez Gene: 94025 Human
- Omim: 606154 Human
- SwissProt: Q8WXI7 Human
- Unigene: 432676 Human
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別名
- CA 125 antibody
- CA-125 antibody
- CA125 antibody
see all
画像
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Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab693 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab693, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [OC125] (ab693)
Paraffin embedded human ovarian cancer tissue stained for MUC16 with ab693 (1/50 dilution) in immunohistochemical analysis.
プロトコール
参考文献 (12)
ab693 は 12 報の論文で使用されています。
- Pantshwa JM et al. Chemotherapeutic Efficacy of Implantable Antineoplastic-Treatment Protocols in an Optimal Mouse Model for Human Ovarian Carcinoma Cell Targeting. Int J Mol Sci 19:N/A (2018). PubMed: 30287783
- Kline JB et al. Tumor antigen CA125 suppresses antibody-dependent cellular cytotoxicity (ADCC) via direct antibody binding and suppressed Fc-? receptor engagement. Oncotarget 8:52045-52060 (2017). Flow Cyt ; Human . PubMed: 28881712
- Kim BR et al. SOX2 and PI3K Cooperate to Induce and Stabilize a Squamous-Committed Stem Cell Injury State during Lung Squamous Cell Carcinoma Pathogenesis. PLoS Biol 14:e1002581 (2016). ICC/IF ; Human . PubMed: 27880766
- Zeng WJ et al. Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification. Int J Nanomedicine 10:1759-68 (2015). IHC-P ; Human . PubMed: 25784803
- Zhang J et al. Megakaryocytic potentiating factor and mature mesothelin stimulate the growth of a lung cancer cell line in the peritoneal cavity of mice. PLoS One 9:e104388 (2014). Flow Cyt ; Mouse . PubMed: 25118887