Anti-MUC1 抗体 [E29] (ab6261)

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Key features and details

  • Mouse monoclonal [E29] to MUC1
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-MUC1 antibody [E29]
    MUC1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [E29] to MUC1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, IHC-Frmore details

  • 種交差性

    交差種: Human

  • 免疫原

    BALB/C mice were immunized with delipidated extract of human cream.

  • ポジティブ･コントロール

    • Breast carcinoma.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
IHC-Fr
  • 追加情報
    IHC-P: 1/25 - 1/50 (30 min, RT). Perform enzymatic antigen retrieval before commencing with IHC staining protocol (Enzymatic predigestion of paraffin embedded tissues is not necessary but in some cases may enhance the immunostaining).
    IHC-Fr: 1/25 - 1/50 (30 min, RT).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 機能

      The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
      The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

    • 組織特異性

      Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.

    • 関連疾患

      MUC1/CA 15-3 is used as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence (PubMed:20816948). Decreased levels over time may be indicative of a positive response to treatment. Conversely, increased levels may indicate disease progression. At an early stage disease, only 21% of patients exhibit high MUC1/CA 15-3 levels, that is why CA 15-3 is not a useful screening test. Most antibodies target the highly immunodominant core peptide domain of 20 amino acid (APDTRPAPGSTAPPAHGVTS) tandem repeats. Some antibodies recognize glycosylated epitopes.
      Medullary cystic kidney disease 1

    • 配列類似性

      Contains 1 SEA domain.

    • 発生段階

      During fetal development, expressed at low levels in the colonic epithelium from 13 weeks of gestation.

    • 翻訳後修飾

      Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM.
      Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17.
      Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane.
      Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1.
      The N-terminal sequence has been shown to begin at position 24 or 28.

    • 細胞内局在

      Secreted; Cell membrane. Cytoplasm. Nucleus. On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus and Apical cell membrane. Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
    • Information by UniProt

    • 参照データベース

      • 別名

        • ADMCKD antibody
        • ADMCKD1 antibody
        • Breast carcinoma associated antigen DF3 antibody
        • Breast carcinoma-associated antigen DF3 antibody
        • CA 15-3 antibody
        • CA15 3 antibody
        • CA15 3 antigen antibody
        • CA15-3 antibody
        • CA15.3 antibody
        • Cancer antigen 15-3 antibody
        • Carcinoma associated mucin antibody
        • Carcinoma-associated mucin antibody
        • CD 227 antibody
        • CD227 antibody
        • DF3 antigen antibody
        • EMA antibody
        • Episialin antibody
        • Epithelial Membrane Antigen antibody
        • H23 antigen antibody
        • H23AG antibody
        • KL 6 antibody
        • KL-6 antibody
        • KL6 antibody
        • Krebs von den Lungen-6 antibody
        • MAM 6 antibody
        • MAM6 antibody
        • MCD antibody
        • MCKD antibody
        • MCKD1 antibody
        • Medullary cystic kidney disease 1 (autosomal dominant) antibody
        • Medullary cystic kidney disease, autosomal dominant antibody
        • MUC 1 antibody
        • MUC-1 antibody
        • MUC-1/SEC antibody
        • MUC-1/X antibody
        • MUC1 antibody
        • MUC1-alpha antibody
        • MUC1-beta antibody
        • MUC1-CT antibody
        • MUC1-NT antibody
        • MUC1/ZD antibody
        • MUC1_HUMAN antibody
        • Mucin 1 antibody
        • Mucin 1 cell surface associated antibody
        • Mucin 1 transmembrane antibody
        • Mucin 1, cell surface associated antibody
        • Mucin-1 subunit beta antibody
        • Peanut reactive urinary mucin antibody
        • Peanut-reactive urinary mucin antibody
        • PEM antibody
        • PEMT antibody
        • Polymorphic epithelial mucin antibody
        • PUM antibody
        • Tumor associated epithelial membrane antigen antibody
        • Tumor associated epithelial mucin antibody
        • Tumor associated mucin antibody
        • Tumor-associated epithelial membrane antigen antibody
        • Tumor-associated mucin antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [E29] (ab6261)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [E29] (ab6261)
        ab6261 staining MUC1 in formalin-fixed, paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

      参考文献 (0)

      ab6261 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab6261 は論文での使用が確認できていません。

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      Question

      I found out that you sell a large number of antibodies against the human cell surface antigen MUC1. Does any of the ones you sell react with the extracellular domain further out than the cleavage site (or with the cleavage site)? Also, do they react with the mature form (several of the muc1 abs only react with low glycosylated precursors)? And thirdly, do they react with rhesus monkey MUC1?

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      Answer

      Thank you for your enquiry regarding our MUC1 antibodies. There is indeed a large selection of those antibodies available in our catalogue and I have summarised below the information we have regarding the epitope recognised and whether we know if the antibody recognises various glycosylated forms of the protein. The extracellular part of the human protein is located with aa24-1158. Many antibodies recognize The APDTR repeat found in this region as you will see below. None of those antibodies have been tested against Macaca mulatta tissue, and a homology search showed 78% homology between the human protein and the monkey protein which may be low for using monoclonal antibodies. Ab8323- Binds with high efficiency to epitope within the VNTR tandem repeat peptide region of MUC1 molecule. Recognizes underglycosylated and natural MUC1 protein. Ab696. epitope not mapped. This antibody is specific to 300 kD DF3 antigen. Ab14690. Immunogen sequence is unknown (the antibody is not made by Abcam and kept confidential from us). It is a “60 aa long synthetic peptide representing a 20 amino acid repeat sequence from mucin 1". The antibody labels underglycosylated mucin. 15481. Immunogen sequence is unknown (the antibody is not made by Abcam and kept confidential from us). We know the immunogen is from the cytoplasmic tail part of the protein. Type of underglycosylated or mature protein recognized: unknown. Ab8665: It recognizes the multiple protein epitope that is relatively insensitive to glycosylation. Therefore, it is very sensitive in the detection of episialin expression in different epithelial tissues. Multiple epitopes. Ab22711. SM3 antibody recognises the under-glycosylated form of MUC1. Epitope not mapped. Ab8605. epitope not mapped. Type of underglycosylated or mature protein recognized: unknown. Ab8618. The dominant epitope of this antibody is the 5-mer PDTRP of MUC1 tandem repeat as established with “epitope fingerprinting.” Type of underglycosylated or mature protein recognized: unknown. Ab8606: The dominant epitope of this antibody is the 12-mer GVTSAPDTRPAP of the MUC1 tandem repeat as established with “epitope fingerprinting”. Type of underglycosylated or mature protein recognized: unknown. Ab8608. The dominant epitope of this antibody is the 7mer TSAPDTR of the MUC1 tandem repeat as established with epitope fingerprinting. Type of underglycosylated or mature protein recognized: unknown. Ab8607: The dominant epitope of this antibody is APDTR as established with “epitope fingerprinting.” Type of underglycosylated or mature protein recognized: unknown. Ab8322, ab10115, ab22754, ab22751. Mabs bind with high efficiency with different regions of VTSAPDTRPAPGSTAPPAHGVTSA synthetic peptide spanning the one repeat of VNTR extracellular portion of MUC1 molecule. They react with VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosylated MUC1 prepared from tumor fluids or as a result of chemical treatment of human milk MUC1. These Mabs do not recognize natural MUC1 protein isolated from human milk by affinity purification on carbohydrate epitope specific Mabs. Ab10118, ab10119 Mabs bind with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conformations and peptide- carbohydrate compositions. Ab efficiently recognize underglycosylated and natural MUC1 protein isolated from human milk by affinity chromatography. Ab10120. Mabs bind with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conformations and peptide- carbohydrate compositions. Mab M4H2 reacts with VNTR20 recombinant unglycosylated fragment of MUC1 protein (and very weakly with monomeric MUC1 peptide), with deglycosylated and underglycosylated natural MUC1 (but not with completely glycosylated natural milk MUC1). Ab10124. epitope: peptide repeat RPAP region. Type of underglycosylated or mature protein recognized: unknown. Ab10114. epitope: Peptide tandem repeat in MUC1 core. Type of underglycosylated or mature protein recognized: unknown. Ab6261, ab853. EPitope: unknow. This antibody reacts with an antigen of 265-400 kD belonging to a heterogeneous group of heavily glycosylated proteins called human milk fat globule proteins. I hope this information helps, please do not hesitate to contact us if you need any more advice or information to help you choose the correct antibody suitable for your needs.

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      Question

      SO wou know the concentration of this antibody? It is listed as purified. Also, can you suggest a MUC1 antibody that is useful for labeling of liposomes or micorspheres?

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      Answer

      The concentration is approx. 100 ug/ml.

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