Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [UMB3] to Mu Opioid Receptor - C-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
製品名Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal
Mu Opioid Receptor 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [UMB3] to Mu Opioid Receptor - C-terminal
The antibody detects the band of interest in transfected cell lines. When tested in our lab, we were not able to detect any signal from the endogenous protein.
This antibody can be used in IHC and ICC/IF as demonstrated in several publications, however, it did not pass our internal validation in these applications. Please refer to PMID 20851148, 21957251, 26290245, and other references.
アプリケーション適用あり: WBmore details
適用なし: Flow Cyt
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human Mu Opioid Receptor aa 350-450 (C terminal). The exact sequence is proprietary.
Database link: P35372
- WB: Mu Opioid Receptor transfected HEK-293 lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab134054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 75-78 kDa (predicted molecular weight: 45 kDa).|
機能Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin.
配列類似性Belongs to the G-protein coupled receptor 1 family.
- Information by UniProt
- hMOP antibody
- LMOR antibody
- M-OR-1 antibody
All lanes : Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054) at 1/1000 dilution
Lane 1 : Mu Opioid Receptor transfected HEK-293 (Human epithelial cell line from embryonic kidney) lysate
Lane 2 : HEK-293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
Immunocytochemistry of rat MOR-transfected HEK-293 cells. Cells were exposed for 30 min to etonitazene (D) or left untreated (C), subsequently fixed and immunofluorescently stained with ab134054. Note that in untreated cells prominent immunofluorescence was localized at the level of the plasma membrane and that etonitazene exposure induced a rapid translocation of MOR receptors from the plasma membrane into the cytosol.
For more protocol information please see PMID: 20851148.
ab134054 は 25 報の論文で使用されています。
- DiCello JJ et al. Mu and Delta Opioid Receptors Are Coexpressed and Functionally Interact in the Enteric Nervous System of the Mouse Colon. Cell Mol Gastroenterol Hepatol 9:465-483 (2020). PubMed: 31759144
- Zhang XY et al. Mu-Opioid Receptors Expressed in Glutamatergic Neurons are Essential for Morphine Withdrawal. Neurosci Bull N/A:N/A (2020). PubMed: 32451910
- Arttamangkul S et al. Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry. Elife 8:N/A (2019). PubMed: 31589142
- Popova D et al. Synaptic Regulation by OPRM1 Variants in Reward Neurocircuitry. J Neurosci 39:5685-5696 (2019). PubMed: 31109961
- Ehrlich AT et al. Biased Signaling of the Mu Opioid Receptor Revealed in Native Neurons. iScience 14:47-57 (2019). PubMed: 30925410