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Anti-Mu Opioid Receptor 抗体 (ab17934)

Reviews (1)Q&A (6)References (7)
Western blot - Anti-Mu Opioid Receptor antibody (ab17934)
  • Immunocytochemistry - Anti-Mu Opioid Receptor antibody (ab17934)

Key features and details

  • Rabbit polyclonal to Mu Opioid Receptor
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Mu Opioid Receptor antibody [UMB3] - Low endotoxin, Azide free (ab227067)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Mu Opioid Receptor antibody
    Mu Opioid Receptor 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Mu Opioid Receptor

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC, WBmore details

  • 種交差性

    交差種: Mouse, Rat
    交差が予測される動物種: Human

  • 免疫原

    Synthetic peptide (Human) (Internal sequence,

    ARIRQNTREHPSTAC

    ).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: Rat brain lysates. ICC: Neuro-2A cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab17934の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
1/250.
WB (1)
1/1000. Predicted molecular weight: 45 kDa.
特記事項
ICC
1/250.
WB
1/1000. Predicted molecular weight: 45 kDa.

ターゲット情報

画像

  • Western blot - Anti-Mu Opioid Receptor antibody (ab17934)
    Western blot - Anti-Mu Opioid Receptor antibody (ab17934)
    Anti-Mu Opioid Receptor antibody (ab17934) at 1/1000 dilution + Rat brain lysates

    Secondary
    Goat anti-rabbit IgG HRP conjugate.

    Predicted band size: 45 kDa



     

    SDS-PAGE on a 10% Tris-glycine gel

  • Immunocytochemistry - Anti-Mu Opioid Receptor antibody (ab17934)
    Immunocytochemistry - Anti-Mu Opioid Receptor antibody (ab17934)

    Neuro-2A cells stained for Mu Opioid Receptor (green) using ab17934 at 1/250 dilution in ICC/IF. It was followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control.

参考文献 (7)

ab17934 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab17934 は 7 報の論文で使用されています。

  • Zhang JJ  et al. Inhibition of mu-opioid receptor suppresses proliferation of hepatocellular carcinoma cells via CD147-p53-MAPK cascade signaling pathway. Am J Transl Res 13:3967-3986 (2021). PubMed: 34149993
  • Blackwood CA  et al. Molecular Adaptations in the Rat Dorsal Striatum and Hippocampus Following Abstinence-Induced Incubation of Drug Seeking After Escalated Oxycodone Self-Administration. Mol Neurobiol 56:3603-3615 (2019). PubMed: 30155791
  • Seoane-Collazo P  et al. Central nicotine induces browning through hypothalamic ? opioid receptor. Nat Commun 10:4037 (2019). PubMed: 31492869
  • Blackwood CA  et al. Escalated Oxycodone Self-Administration and Punishment: Differential Expression of Opioid Receptors and Immediate Early Genes in the Rat Dorsal Striatum and Prefrontal Cortex. Front Neurosci 13:1392 (2019). PubMed: 31998063
  • Hubbs AF  et al. Accumulation of Ubiquitin and Sequestosome-1 Implicate Protein Damage in Diacetyl-Induced Cytotoxicity. Am J Pathol N/A:N/A (2016). ICC/IF ; Mouse . PubMed: 27643531
  • Leoncikas V  et al. Generation of 2,000 breast cancer metabolic landscapes reveals a poor prognosis group with active serotonin production. Sci Rep 6:19771 (2016). PubMed: 26813959
  • Niwa H  et al. Evaluation of primary opioid receptor antibodies for use in western blotting. Br J Anaesth 108:530-2 (2012). WB ; Rat . PubMed: 22337965

レビューと Q&A

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1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-Mu Opioid Receptor antibody

 Poor
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cells)
Loading amount
80 µg
Specification
HEK293 cells
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

投稿 Nov 01 2010

Question

A quien corresponda,
Estoy interesada en un anticuerpo anti-receptor mu-opioide de rata que reconozca un epitope extracelular. He encontrado dos anticuerpos en el sitio web que reconocen el extremo N-terminal, pero del receptor humano, y hay otros dos, que reconocen rata, pero cuyos inmunógenos son péptidos sintéticos de os cuales no se aclara exactamente la posición.
La información que se detalla es la siguiente:
Anti-Mu Opioid Receptor antibody (ab17934)
Immunogen
Synthetic peptide (Human) (Internal sequence, ARIRQNTREHPSTAC).
ARIRQNTREHPSTAC
Anti-Mu Opioid Receptor antibody (ab64746)
Immunogen
Synthetic peptide: NHQLENLEAETAPLP
NHQLENLEAETAPLP
Le estaría sumamente agradecida si pudieran por favor indicarme si alguno de estos dos anticuerpos sería válido para mi objetivo.
Muchas gracias desde ya y un cordial saludo,

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Answer

Gracias por contactarnos.
Después de consultar este caso con mis compañeros del laboratorio, acerca de los siguientes anticuerpos contra Mu Opioid Receptor validados en tejidos de rata: ab10275, ab17934, ab64746, ab128013 y ab134054, me temo que ninguno de ellos ha demostrado reconocer el epítopo extracelular de la proteína.
Todos ellos se han generado a partir del extremo C terminal de la proteína, por lo que, a pesar de no haberse mapeado el epítopo que reconocen, probablemente detecten el dominio citoplásmico de la misma.
La solución que te propongo es testar uno de los anticuerpos que hay en el catálogo hecho a partir del extremo N terminal de la proteína, y testarlo en rata. Tenemos en abcam un sistema de evaluación de anticuerpos por el cual al testar uno de ellos en una aplicación o especie que no se ha testado, puedes beneficiarte de un descuento en tu próxima compra por valor del anticuerpo, si lo usas en esa especie o aplicación nueva y nos mandas los resultados a la web en forma de Abreview.
Para saber más sobre este sistema: www.abcam.com/abtrial
De las posibles alternativas, ab140911, ab137460 o ab137277 indícame si quieres cuál os interesaría más, para averiguar su homología con la proteína en rata y determinar la posibilidad de reaccionar en esta especie, en caso de que queráis participar en el sistema Abtrial.
Espero haberte ayudado. No dudes en contactarnos de nuevo para cualquier otra consulta.

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Question

We have tried using this receptor antibody, along with Rab5 to investigate Mu opioid receptor trafficking in rat brain tissue sections.
The rats have been treated with either saline or two compounds dissolved in saline by IP injection.
The brains have then been removed and snap frozen using isopentane and stored in the -80deg freezer before sectioned in a crystat to 20um thick.
We have tried optimizing fixation (PFA, acetone, methanol), permeableisation (triton-x, tween, saponin), antibody concentration (primary and secondary) and duration/temperature, blocking (times and concentration of blocking).
We have ascertained good staining in thalamus where Mu opioid receptors are abundantly expressed on interneurons and cortex where mu expressed, but to a lesser degree to that in thalamus.
However, we have only observed green (mu secondary = fluoro488) staining consistent with astrocytic/glail neurons, no interneurons or pyramidal neurons are stained.
We would expect little or no astroglial mu opioid receptors in thalamus or cortex.
We were wondering if you might be able to shed some light on what this staining might be specific to on these cells and how we might go about getting some specific mu opioid receptor staining in actually neurons.
Image: green = mu 488, Rab5 594 = red, blue = DAPI

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Answer

Thank you for contacting us.
We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and this antibody (as indicated on the datasheet) has not been tested for IHC. We are therefore unable to provide any specific recommendation or apply our guarantee for this particular product in this specific application.
We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests.
I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

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Question

Problem: Antibody not working in WB Primary was used at 1:1000 and 1:500 dilutions, overnight incubation at +4C, in 5% skim milk in TBST (tween 0.1%). The secondary antibody is a donkey anti-rabbit HRP conjugated from GE Healthcare used at 1:20000, routinely used in our lab. The samples are membranes from CHO cells transfected with rat MOR-1 and one splice variant of it that has also the same epitope (both should be recognized, but none is ). Samples from mock tranfected CHOs, rat MOR-1 transfected CHOs and rat MOR-1 variant transfected CHOs were probed in parallel also with another rabbit polyclonal (this one working fine) that is showing that our receptors are indeed expressed. The gel was run in non reducing conditions, membranes were extracted in a buffer containing 2mM DTT.

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Answer

Thank you for this information. Although your samples are showing receptor expressio with another product, Abcam antibodies will have been tested under reduced and denatured conditions only and we recommend using reducing and denaturing conditions unless otherwise stated on the datasheet. You may need to change your conditions in order to improve your result with this product. Please let me know if you have any further questions.

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Question

The customer has used this ab before and one batch worked and other didn't. Currenlythere seems to be shortage of units - the customer would like to know when we expect to get more into stock - and will this be the same batch? He enquired in June and has yet to receive a reply.

Read More
Answer

Thank you for stopping by the Abcam booth at SFN, my colleague Sarah passed your enquiry onto me. I'm sorry that you didn't receive our response back in June. Presently, ab17934 is currently in stock. The batches that we have are batches 129573, 129620, and 132645. What was the batch number that you experienced problems with? Please let me know if you have any additional questions, and enjoy the rest of the conference!

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Question

BATCH NUMBER 111484 ORDER NUMBER 05051655 DESCRIPTION OF THE PROBLEM High background SAMPLE (1) WCL isolated from mu-Oproid receptor expressed cell lines (2) WCL isolated from wild type of cultured HEK cell (3) immunoprecipitated proteins PRIMARY ANTIBODY Dilution: 1:1000, Incubation time: overnight Incubation temprature: 4 degrees DETECTION METHOD ECL system from Amersham POSITIVE AND NEGATIVE CONTROLS USED Positive: recombinant protein of mouse mu-Opoid receptor Negative: WCL isolated from wild type HEK cell ANTIBODY STORAGE CONDITIONS this antibody was stored at -20 degrees. AMOUNT OF PROTEIN LOADED The amount of protein being loaded per lane : 20ug/ lane TRANSFER AND BLOCKING CONDITIONS for 30 minutes at room temp in Block Ace SECONDARY ANTIBODY Anti rabbit IgG-HRP conjugated from Amersham This secondary antibody is working well using other primary antibodies. ADDITIONAL NOTES This is the inquiry from Cosmo. The HA tag antibody the customer has is working well in these systems. Would you please let me know your advice?

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Answer

Thank you for your enquiry and for taking your time to fill in the on-line Questionnaire. As we understand from your e-mail, the customer used mouse Mu Opioid Receptor expressing HEK cells. We would like to emphasize that this antibody was rtaised against synthetic peptide of human Mu Opioid Receptor. Therefore the antibody recognizes human protein, but unfortunately other species i.e. mouse has not been tested for cross-reaction. Although, there is a chance that it may work in this species due to 94% sequence homology. 1. We would suggest running a good positive control along with the samples, lysate from human brain or spinal cord would be a good candidate. 2. We do not have information about the Bock Ace, so would rather suggest using 5% BSA-TBST for blocking. 3. Please make sure that the customer use RIPA buffer for sample preparation: RIPA Base Ingredients Tris-HCl: 50 mM, pH 7.4 NP-40: 1% Na-deoxycholate: 0.25% NaCl: 150 mM EDTA: 1 mM PMSF: 1 mM Aprotinin, leupeptin, pepstatin: 1 microgram/ml each RIPA Protease Inhibitors Phenylmethylsulfonyl fluoride (PMSF) (200 mM stock solution in isopropanol; store at room temperature) EDTA (calcium chelator; 100 mM stock solution in H2O, pH 7.4) Leupeptin (store frozen in aliquots, 1 mg/ml in H2O) Aprotinin (store frozen in aliquots, 1 mg/ml in H2O) Pepstatin (store frozen in aliquots, 1 mg/ml in methanol) We hope this information will be useful. Should your customer still have problem, then please do not hesitate to contact us again.

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Question

The customer would like to know the peptide sequence that produced this antibody.

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Answer

Thank you for your enquiry. The peptide sequence is :Ac-Ala-Arg-Ile-Arg-Gln-Asn-Thr-Arg-Glu-His-Pro-Ser-Thr-Ala-Cys-NH2 and this information has been added to the datasheet. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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