Anti-mTOR (phospho S2448) 抗体 (ab1093)
製品の概要
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製品名Anti-mTOR (phospho S2448) antibody
mTOR 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to mTOR (phospho S2448)
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由来種Rabbit
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アプリケーション適用あり: IHC-P, WB, ELISAmore details
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種交差性交差種: Human
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免疫原
Synthetic peptide: KRSRTRTDpSYSAGQSVEI, corresponding to C terminal amino acids 2440-2463 of Human mTOR.
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ポジティブ･コントロール
- serum starved 293T cell lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファー0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% Sodium Azide, pH 7.2
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Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab1093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
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| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | 1/1000. Detects a band of approximately 250 kDa (predicted molecular weight: 288 kDa). | |
| ELISA | 1/6000 - 1/29000. Assayed aginst 0.1 µg of the immunizing peptide and has not been tested against the endogenous protein. |
ターゲット情報
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機能Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
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組織特異性Expressed in numerous tissues, with highest levels in testis.
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配列類似性Belongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 7 HEAT repeats.
Contains 1 PI3K/PI4K domain. -
翻訳後修飾Autophosphorylated; when part of mTORC1 or mTORC2.
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細胞内局在Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
- Information by UniProt
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参照データベース
- Entrez Gene: 2475 Human
- Omim: 601231 Human
- SwissProt: P42345 Human
- Unigene: 338207 Human
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別名
- dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
- FK506 binding protein 12 rapamycin associated protein 1 antibody
- FK506 binding protein 12 rapamycin associated protein 2 antibody
see all
画像
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Western blot - Anti-mTOR (phospho S2448) antibody (ab1093)This image is courtesy of Angela Carter, Experimental Therapeutics, Ontario Cancer Inst, Toronto, CanadaRabbit polyclonal to phospho mTOR (Ser 2448) at 1/1000 on 293T cell extract shows a band at approx 250 kDa.
Cells were serum starved for 24 hours:Lane 1 : control
Lane 2 : treated with IGF-1 (100 ng/ml) for 20 minRabbit polyclonal to phospho mTOR (Ser 2448) at 1/1000 on 293T cell extract shows a band at approx 250 kDa. Cells were serum starved for 24 hours:
Lane 1 : control
Lane 2 : treated with IGF-1 (100 ng/ml) for 20 min -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody (ab1093)ab1093 at 5 µg/ml staining proximal convulted tubules of the kidney. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
参考文献
This product has been referenced in:
- Thongon N et al. Cancer cell metabolic plasticity allows resistance to NAMPT inhibition but invariably induces dependence on LDHA. Cancer Metab 6:1 (2018). Read more (PubMed: 29541451) »
- Ke K & Lou T MicroRNA-10a suppresses breast cancer progression via PI3K/Akt/mTOR pathway. Oncol Lett 14:5994-6000 (2017). Read more (PubMed: 29113237) »
レビューと Q&A
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