Anti-mTOR 抗体 (ab2732)

Thank you and your customer for your inquiry.

I believe that the images that your customers provided do show very nice specific staining.

According to the Uniprot homepage (http://www.uniprot.org/uniprot/P42345) mTOR is expressed in the endoplasmic reticulum membrane, the Golgi apparatus membrane, the mitochondrion outer membrane, theLysosome and the Cytoplasm. It shuttles between cytoplasm and nucleus.

If it is in the nucleus, it is located in thePML bodies which is nicely shown by your customers image.

I am happy to confirm that this antibody does not seem to exhibit any un-specific staining and that your customers results are very nice.

I hope I could reassure your customer that their results are specific. Please let me know if your customer has any other questions.

Thank you for contacting us.

As we deliver 50 µg at 1mg/ml, the expected volume should be 50ul.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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I am glad you have found the information I provided of help. If I can be of any further assistance, please do not hesitate to let me know.

Until then, I wish you all the best with your research.

Thank you for contacting us and your interest in our products.

When trying to detect a protein of the size of mTOR in Western blotting, there is some movement of the band that would be expected away from the predicted band size. Due to how the ladder and protein runs in the SDS-PAGE gel there can be quite large variations in proteins of this size so the size of 255 kDa observed with ab2732 would not be unexpected. The antibody has been used in the staining of HepG2 cells as well as rat testis, supporting it detecting the expected protein.

I have had a look through the antibodies we have against this target and I would suggest also taking a look at the mouse monoclonal ab87540 which has been used in Western blotting and produced a clean band of ˜268 kDa with HEK293 and human liver tissue (amongst other samples).

However, I'd like to reassure you that both the ab2732 and ab87540 would be covered by the Abpromise guarantee if you intend to use it in Western blotting with human samples. More information on this guarantee can be found from the following link:

https://www.abcam.com/abpromise

I'd also like to draw your attention to our protocol booklet which has some useful advice when performing Western blotting with such large proteins. This booklet can be accessed from the following link:

https://www.abcam.com/index.html?pageconfig=resource&rid=13111

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

Thank you for your email.
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