Application
Western blot
Sample
Human Cell lysate - other (U2OS Cells - Cytosol v Membrane Fractionation)
Gel Running Conditions
Reduced Denaturing (4-20% Acrylamide)
Loading amount
30 µg
Specification
U2OS Cells - Cytosol v Membrane Fractionation
Blocking step
Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/1000
Secondary antibody
Dilution
1/2500
Name
Non-Abcam antibody was used: Bio-Rad StarBright Blue 700
Host species: Goat
Clonality: Polyclonal
Conjugation: Other
Host species: Goat
Clonality: Polyclonal
Conjugation: Other
Detection
Detection method
Chemidoc
Negative control
Cytosolic fraction (Cyt)
Exposure
30 second(s)
Bands
Specific: 20 kDa
Positive control
Input from U2OS cells following removal of the nuclear fraction and membrane fraction (Mem)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Michael Munson
Verified customer
投稿 Sep 08 2017