Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (15% GEL)
Sample
Human Cell lysate - whole cell (H460)
Specification
H460
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: GOAT ANTI-MOUSE
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: 20 kDa Non-specific: 40, 45 kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
Additional Notes
Difficult to assess as the antibody does not even label mitochondria
Abcam response
Thank you for your review. I agree it is difficult to assess whether the band at ~45 kD is the MTCO2 band running low, or whether it is non-specific. Please be advised that this product is covered by our Abpromise guarantee and we can provide a replacement, credit or refund if the purchase was within the last 12 months.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 26 2014