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  1. Link

    mtco1-antibody-1d6e1a8-ab14705.pdf

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Anti-MTCO1 抗体 [1D6E1A8] (ab14705)

  • Datasheet
  • SDS
Reviews (41)Q&A (27)References (409)

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Immunocytochemistry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

Key features and details

  • Mouse monoclonal [1D6E1A8] to MTCO1
  • Suitable for: ICC, IHC-P, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647

こちらの製品もご検討ください

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Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
一次抗体
Product image
Anti-MTCO1 antibody [EPR19628] (ab203912)

関連製品

製品の概要

  • 製品名

    Anti-MTCO1 antibody [1D6E1A8]
    MTCO1 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [1D6E1A8] to MTCO1
  • 由来種

    Mouse
  • 特異性

    In mouse liver lysate a specific band below 37 kDa was detected.
  • アプリケーション

    適用あり: ICC, IHC-P, WB, Flow Cytmore details
  • 種交差性

    交差種: Mouse, Rat, Cow, Human
    交差が予測される動物種: Sheep, Goat, Cat, Dog, Pig, Caenorhabditis elegans, Zebrafish, Quail, Rhesus monkey, Chinese hamster, Common marmoset
  • 免疫原

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • IHC-P: Human cerebellar and colon tissue. WB: Mouse, human, bovine and rat heart mitochondria lysate. Flow Cyt: HEK-293 cells. ICC: HeLa cells.
  • 特記事項

    Western blot protocol advice:
    For best results with this antibody in Western blot, do not boil samples before loading onto the gel. Boiling of the sample will cause a loss of signal.

    Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.

    This antibody clone [1D6E1A8] is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C.
  • バッファー

    pH: 7.5
    Preservative: 0.02% Sodium azide
    Constituent: HEPES buffered saline
  • Concentration information loading...
  • 精製度

    Ammonium Sulphate Precipitation
  • 特記事項(精製)

    Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • ポリ/モノ

    モノクローナル
  • クローン名

    1D6E1A8
  • アイソタイプ

    IgG2a
  • 軽鎖の種類

    kappa
  • 研究分野

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex IV
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] (ab154477)
    • Alexa Fluor® 647 Anti-MTCO1 antibody [1D6E1A8] (ab198600)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Positive Controls

    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab14705の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC (2)
Use a concentration of 1 µg/ml.
IHC-P (13)
Use a concentration of 5 µg/ml.
WB (18)
Use a concentration of 0.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 57 kDa).
Flow Cyt (1)
Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

特記事項
ICC
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 5 µg/ml.
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 57 kDa).
Flow Cyt
Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.
  • パスウェイ

    Energy metabolism; oxidative phosphorylation.
  • 関連疾患

    Defects in MT-CO1 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-CO1 are a cause of anemia sideroblastic acquired idiopathic (AISA) [MIM:516030]; a disease characterized by inadequate formation of heme and excessive accumulation of iron in mitochondria.
    Defects in MT-CO1 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    Defects in MT-CO1 are associated with recurrent myoglobinuria mitochondrial (RM-MT) [MIM:550500]. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness, and followed by excretion of myoglobin in the urine.
    Defects in MT-CO1 are a cause of deafness sensorineural mitochondrial (DFNM) [MIM:500008]. DFNM is a form of non-syndromic deafness with maternal inheritance. Affected individuals manifest progressive, postlingual, sensorineural hearing loss involving high frequencies.
    Defects in MT-CO1 are a cause of colorectal cancer (CRC) [MIM:114500].
  • 配列類似性

    Belongs to the heme-copper respiratory oxidase family.
  • 細胞内局在

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession P00395 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 2565700 Caenorhabditis elegans
    • Entrez Gene: 281919 Cow
    • Entrez Gene: 4512 Human
    • Entrez Gene: 17708 Mouse
    • Entrez Gene: 26195 Rat
    • Entrez Gene: 140539 Zebrafish
    • Omim: 516030 Human
    • SwissProt: P24893 Caenorhabditis elegans
    • SwissProt: P00396 Cow
    • SwissProt: Q9ZZ64 Dog
    • SwissProt: P00395 Human
    • SwissProt: P00397 Mouse
    • SwissProt: O79876 Pig
    • SwissProt: P05503 Rat
    • SwissProt: O78749 Sheep
    • SwissProt: Q9MIY8 Zebrafish
    see all
  • 別名

    • COI antibody
    • COX I antibody
    • COX1 antibody
    • COX1_HUMAN antibody
    • COXI antibody
    • Cytochrome c oxidase polypeptide I antibody
    • Cytochrome c oxidase subunit 1 antibody
    • Cytochrome C Oxidase subunit I antibody
    • Mitochondrially encoded cytochrome c oxidase I antibody
    • MT CO1 antibody
    • MT-CO1 antibody
    • MTCO 1 antibody
    • MTCO1 antibody
    see all

画像

  • Immunocytochemistry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunocytochemistry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    ab14705 staining MTCO1 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab14705 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 100% methanol (5 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    All lanes : Anti-MTCO1 antibody [1D6E1A8] (ab14705) at 0.5 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 5 µg

    Secondary
    All lanes : Goat Anti-Mouse IgG

    Predicted band size: 57 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?
    Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.



    Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)

    IHC image of MTCO1 staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14705, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Overlay histogram showing HEK293 cells stained with ab14705 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14705, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)Image from Phillips J et al., Sci Rep. 2016 May 16;6:26013. Fig 3b doi: 10.1038/srep26013. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    250 μm human cerebellar sections from control individuals and a patient with mitochondrial disease underwent passive clearing at 37 °C for 2 or 4 weeks.

    The quality of immunofluorescent staining is determined by duration of passive clearing; 2 weeks of passive clearing produced minimal labelling of the white matter in the granule cell layer (NF-H; green; 488 nm and MBP; red, 546 nm) with an absence of labelling of mitochondria (MTCO1 (COXI) (ab14705, 1/100); purple; 647 nm; Extending passive clearing to 4 weeks improved the quality of stain with identifiable Purkinje cells and their axons (NF-H, green; 488 nm) and their myelin sheaths (MBP; red, 546 nm) and mitochondria (MTCO1 (COXI) (ab14705, 1/100); purple; 647 nm.

プロトコール

  • Mouse on Mouse staining protocol

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (409)

ab14705 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab14705 は 409 報の論文で使用されています。

  • He Q  et al. Mtu1 defects are correlated with reduced osteogenic differentiation. Cell Death Dis 12:61 (2021). PubMed: 33431792
  • Iwasaki H  et al. MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells. Sci Rep 11:1161 (2021). PubMed: 33441918
  • Romani M  et al. NAD+ boosting reduces age-associated amyloidosis and restores mitochondrial homeostasis in muscle. Cell Rep 34:108660 (2021). PubMed: 33472069
  • Yagi M  et al. Mitochondrial translation deficiency impairs NAD+ -mediated lysosomal acidification. EMBO J 40:e105268 (2021). PubMed: 33528041
  • Cardoso S  et al. IGF1R Deficiency Modulates Brain Signaling Pathways and Disturbs Mitochondria and Redox Homeostasis. Biomedicines 9:N/A (2021). PubMed: 33562061
View all Publications for this product

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1-10 of 68 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MTCO1 antibody [1D6E1A8]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Common marmoset Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2%
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Sep 15 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MTCO1 antibody [1D6E1A8]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Lamb kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Lamb kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2%
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Sep 15 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MTCO1 antibody [1D6E1A8]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Sep 15 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MTCO1 antibody [1D6E1A8]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Sep 15 2021

Western blot abreview for Anti-MTCO1 antibody [1D6E1A8]

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
60 µg
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Read More

Abcam user community

Verified customer

投稿 Feb 02 2021

Immunohistochemistry (Frozen sections) abreview for Anti-MTCO1 antibody [1D6E1A8]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

投稿 Nov 12 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MTCO1 antibody [1D6E1A8]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA ph 9.0
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Nov 12 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MTCO1 antibody [1D6E1A8]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Nov 11 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MTCO1 antibody [1D6E1A8]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate ph 6
Permeabilization
No
Specification
brain tissue
Blocking step
Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Formaldehyde
Read More

Herr Dr. Markus Kipp

Verified customer

投稿 Aug 21 2018

Western blot abreview for Anti-MTCO1 antibody [1D6E1A8]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (SH-SY5Y)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
15 µg
Specification
SH-SY5Y
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 May 23 2018

1-10 of 68 Abreviews or Q&A

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