Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Ventana Protease 1
Permeabilization
No
Specification
Kidney
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: Ventana antibody diluent
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Ventana Optiview DAB detection kit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
Additional block step added (30 mins in 5% goat serum).
Fixation-dependant staining.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 25 2018