Anti-MTA2/PID 抗体 (ab8106)
Key features and details
- Rabbit polyclonal to MTA2/PID
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MTA2/PID antibody
MTA2/PID 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MTA2/PID -
由来種
Rabbit -
特異性
No cross-reactivity to MTA1. -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human MTA2/PID aa 652-668.
Sequence:PAPSHPASTNEPIVLED
(Peptide available asab6243) -
特記事項
This product was previously labelled as MTA2
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab8106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins. -
組織特異性
Widely expressed. -
配列類似性
Contains 1 BAH domain.
Contains 1 ELM2 domain.
Contains 1 GATA-type zinc finger.
Contains 1 SANT domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9219 Human
- Entrez Gene: 23942 Mouse
- Entrez Gene: 361724 Rat
- Omim: 603947 Human
- SwissProt: O94776 Human
- SwissProt: Q9R190 Mouse
- Unigene: 173043 Human
- Unigene: 25339 Mouse
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別名
- DKFZp686F2281 antibody
- Mata1l1 antibody
- Metastasis associated 1 family member 2 antibody
see all
画像
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Western blot - Anti-MTA2/PID antibody (ab8106)All lanes : Anti-MTA2/PID antibody (ab8106) at 1 µg/ml
Lane 1 : HeLa whole cell lysate with absence of blocking peptide
Lane 2 : HeLa whole cell lysate with presence of blocking peptide
Predicted band size: 75 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody (ab8106)ab8106 at 10µg/ml staining MTA2/PID in Hela cells by ICC/IF
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody (ab8106)ab8106 (2µg/ml) staining MTA2/PID in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody (ab8106)Immunofluorescence of PID in HeLa cells using ab8106 at 10 ug/ml.
データシートおよび資料
参考文献 (21)
ab8106 は 21 報の論文で使用されています。
- Escudero-Paniagua B et al. PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/ß-catenin pathway. Carcinogenesis 41:203-213 (2020). PubMed: 31095674
- Jiang Y et al. Transformable hybrid semiconducting polymer nanozyme for second near-infrared photothermal ferrotherapy. Nat Commun 11:1857 (2020). PubMed: 32312987
- Campbell AE et al. NuRD and CAF-1-mediated silencing of the D4Z4 array is modulated by DUX4-induced MBD3L proteins. Elife 7:N/A (2018). PubMed: 29533181
- Christov CP et al. A NuRD Complex from Xenopus laevis Eggs Is Essential for DNA Replication during Early Embryogenesis. Cell Rep 22:2265-2278 (2018). PubMed: 29490265
- Ma C et al. SALL1 functions as a tumor suppressor in breast cancer by regulating cancer cell senescence and metastasis through the NuRD complex. Mol Cancer 17:78 (2018). PubMed: 29625565
