Anti-MTA2/PID 抗体 (ab8106)
Key features and details
- Rabbit polyclonal to MTA2/PID
- Suitable for: IHC-Fr, ELISA, WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MTA2/PID antibody
MTA2/PID 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MTA2/PID -
由来種
Rabbit -
特異性
No cross-reactivity to MTA1. -
アプリケーション
適用あり: IHC-Fr, ELISA, WB, IHC-P, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8106の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration. PubMed: 18413351
|
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| ELISA |
Use at an assay dependent concentration.
|
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| WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
|
| IP | (1) |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. PubMed: 18413351 |
|
ELISA
Use at an assay dependent concentration. |
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins. -
組織特異性
Widely expressed. -
配列類似性
Contains 1 BAH domain.
Contains 1 ELM2 domain.
Contains 1 GATA-type zinc finger.
Contains 1 SANT domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9219 Human
- Entrez Gene: 23942 Mouse
- Entrez Gene: 361724 Rat
- Omim: 603947 Human
- SwissProt: O94776 Human
- SwissProt: Q9R190 Mouse
- Unigene: 173043 Human
- Unigene: 25339 Mouse
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別名
- DKFZp686F2281 antibody
- Mata1l1 antibody
- Metastasis associated 1 family member 2 antibody
see all
画像
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Western blot - Anti-MTA2/PID antibody (ab8106)All lanes : Anti-MTA2/PID antibody (ab8106) at 1 µg/ml
Lane 1 : HeLa whole cell lysate with absence of blocking peptide
Lane 2 : HeLa whole cell lysate with presence of blocking peptide
Predicted band size: 75 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody (ab8106)ab8106 at 10µg/ml staining MTA2/PID in Hela cells by ICC/IF
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody (ab8106)ab8106 (2µg/ml) staining MTA2/PID in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody (ab8106)Immunofluorescence of PID in HeLa cells using ab8106 at 10 ug/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (26)
ab8106 は 26 報の論文で使用されています。
- Escudero-Paniagua B et al. PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/ß-catenin pathway. Carcinogenesis 41:203-213 (2020). PubMed: 31095674
- Jiang Y et al. Transformable hybrid semiconducting polymer nanozyme for second near-infrared photothermal ferrotherapy. Nat Commun 11:1857 (2020). PubMed: 32312987
- Qian W et al. Metastasis-associated protein 1 promotes epithelial-mesenchymal transition in idiopathic pulmonary fibrosis by up-regulating Snail expression. J Cell Mol Med 24:5998-6007 (2020). PubMed: 32187849
- Heizmann B et al. Ikaros antagonizes DNA binding by STAT5 in pre-B cells. PLoS One 15:e0242211 (2020). PubMed: 33180866
- Marques JG et al. NuRD subunit CHD4 regulates super-enhancer accessibility in rhabdomyosarcoma and represents a general tumor dependency. Elife 9:N/A (2020). PubMed: 32744500
