Anti-MT4-MMP 抗体 [EP1270Y] (ab51075)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1270Y] to MT4-MMP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MT4-MMP antibody [EP1270Y]
MT4-MMP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1270Y] to MT4-MMP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide within Human MT4-MMP aa 150-250 (internal sequence). The exact sequence is proprietary.
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ポジティブ・コントロール
- Mouse brain lysate, human lymphoma tissue or HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1270Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51075の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 67 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/50 - 1/100.
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特記事項 |
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WB
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 67 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/100. |
ターゲット情報
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機能
Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin. -
組織特異性
Expressed in brain, leukocytes, colon, ovary testis and breast cancer. Expressed also in many transformed and non-transformed cell types. -
配列類似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
The precursor is cleaved by a furin endopeptidase. -
細胞内局在
Cell membrane. Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 4326 Human
- Entrez Gene: 23948 Mouse
- Entrez Gene: 288626 Rat
- Omim: 602285 Human
- SwissProt: Q9ULZ9 Human
- SwissProt: Q9R0S3 Mouse
- Unigene: 709245 Human
- Unigene: 42047 Mouse
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別名
- Matrix metalloproteinase 17 antibody
- Matrix metalloproteinase 17 membrane inserted antibody
- Matrix metalloproteinase-17 antibody
see all
画像
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Anti-MT4-MMP antibody [EP1270Y] (ab51075) at 1/1000 dilution + Mouse brain tissue lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 67 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted? -
ab51075 (1:50) staining human MT4-MMP in human lymphoma tissue by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent staining of HeLa cells using ab51075 (1:50).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab51075 は 14 報の論文で使用されています。
- Yan X et al. MT4-MMP promotes invadopodia formation and cell motility in FaDu head and neck cancer cells. Biochem Biophys Res Commun 522:1009-1014 (2020). PubMed: 31813546
- Herber J et al. Click Chemistry-mediated Biotinylation Reveals a Function for the Protease BACE1 in Modulating the Neuronal Surface Glycoproteome. Mol Cell Proteomics 17:1487-1501 (2018). PubMed: 29716987
- Clemente C et al. MT4-MMP deficiency increases patrolling monocyte recruitment to early lesions and accelerates atherosclerosis. Nat Commun 9:910 (2018). PubMed: 29500407
- Yip C et al. MT4-MMP and EGFR expression levels are key biomarkers for breast cancer patient response to chemotherapy and erlotinib. Br J Cancer 116:742-751 (2017). PubMed: 28196064
- Hieronimus B et al. Expression and Characterization of Membrane-Type 4 Matrix Metalloproteinase (MT4-MMP) and its Different Forms in Melanoma. Cell Physiol Biochem 42:198-210 (2017). PubMed: 28531887