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  1. Link

    msin3a-antibody-chip-grade-ab3479.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-mSin3A 抗体 - ChIP Grade (ab3479)

  • Datasheet
  • SDS
Reviews (10) Submit a question References (36)

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Abpromise

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Western blot - Anti-mSin3A antibody - ChIP Grade (ab3479)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody - ChIP Grade (ab3479)
  • ChIP - Anti-mSin3A antibody - ChIP Grade (ab3479)
  • Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)
  • Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)
  • Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody - ChIP Grade (ab3479)

Key features and details

  • Rabbit polyclonal to mSin3A - ChIP Grade
  • Suitable for: ChIP, ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-mSin3A antibody - ChIP Grade
    mSin3A 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to mSin3A - ChIP Grade
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ChIP, ICC/IF, WB, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse mSin3A aa 1-19.
    Sequence:

    MKRRLDDQESDVYAAQQRR


    (Peptide available as ab4995)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Co-factors
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • mSin3A peptide (ab4995)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

Our Abpromise guarantee covers the use of ab3479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration.
ICC/IF 1/50 - 1/500.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 145 kDa).Can be blocked with mSin3A peptide (ab4995).
IHC-P Use a concentration of 2 µg/ml.

ターゲット情報

  • 機能

    Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3A to DNA. Acts as a corepressor for REST.
  • 配列類似性

    Contains 3 PAH (paired amphipathic helix) domains.
  • 翻訳後修飾

    SUMO1 sumoylated by TOPORS. Probably desumoylated by SENP2.
  • 細胞内局在

    Nucleus. Nucleus > nucleolus. Recruited to the nucleolus by SAP30L.
  • Target information above from: UniProt accession Q96ST3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 25942 Human
    • Entrez Gene: 20466 Mouse
    • Omim: 607776 Human
    • SwissProt: Q96ST3 Human
    • SwissProt: Q60520 Mouse
    • Unigene: 513039 Human
    • Unigene: 15755 Mouse
    • 別名

      • AW553200 antibody
      • DKFZP434K2235 antibody
      • FLJ90319 antibody
      • Histone deacetylase complex subunit Sin 3a antibody
      • Histone deacetylase complex subunit Sin3a antibody
      • KIAA0700 antibody
      • Kiaa4126 antibody
      • mKIAA4126 antibody
      • Paired amphipathic helix protein Sin 3a antibody
      • Paired amphipathic helix protein Sin3a antibody
      • Sin 3a antibody
      • SIN3 homolog A antibody
      • SIN3 homolog A transcription regulator (yeast) antibody
      • SIN3 homolog A transcription regulator antibody
      • SIN3 transcription regulator homolog A antibody
      • Sin3a antibody
      • SIN3A protein antibody
      • SIN3A_HUMAN antibody
      • Transcriptional co repressor Sin 3A antibody
      • Transcriptional co repressor Sin3A antibody
      • Transcriptional corepressor Sin3a antibody
      • Transcriptional regulator SIN3A antibody
      see all

    画像

    • Western blot - Anti-mSin3A antibody - ChIP Grade (ab3479)
      Western blot - Anti-mSin3A antibody - ChIP Grade (ab3479)
      Western blot of mSin3A on K562 cell extract using ab3479.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody - ChIP Grade (ab3479)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody - ChIP Grade (ab3479)

      Paraffin-embedded human ovary carcinoma tissue (right panel) stained for mSin3A using ab3479 at 1/200 dilution compared to a negative control without primary antibody (left panel) in immunohistochemical analysis, followed by HRP-conjugated secondary antibody. Detection: DAB staining.

      Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.

    • ChIP - Anti-mSin3A antibody - ChIP Grade (ab3479)
      ChIP - Anti-mSin3A antibody - ChIP Grade (ab3479)

      ChIP analysis of Sin3A was performed using cross-linked chromatin from 1x106 HCT 116 (human colorectal carcinoma cell line) cells treated with serum for 0, 15, and 30 minutes. IP was performed using a multiplex microplate Matrix ChIP assay with 1.0 µl/100 µl well volume of ab3479. Chromatin aliquots from ~1x105 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µl of eluted DNA in 2 µl SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 or exon-2 of Egr1. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. A schematic representation of the Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. 

    • Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)
      Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)

      Immunocytochemical immunoflurescence analysis of NIH-3T3 cells labelling mSin3A using ab3479. Formalin-fixed cells were permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were then incubated with ab3479 in 3% BSA-PBS at a dilution of 1:100 overnight in a 4°C high humidity environment. Cells were then washed with PBST and incubated with a green DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Cells were counterstained with DAPI or Hoechst labelling the nuclear DNA blue. The Left Image is a negative control without the prescence of ab3479. Image magnification is 60X.

    • Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)
      Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)

      Immunocytochemical immunoflurescence analysis of HeLa cells labelling mSin3A using ab3479. Formalin-fixed cells were permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were then incubated with ab3479 in 3% BSA-PBS at a dilution of 1:200 overnight in a 4°C high humidity environment. Cells were then washed with PBST and incubated with a green DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Cells were counterstained blue with DAPI or Hoechst labelling the nuclear DNA and red against actin using an Alexa Fluor® 554 conjugate. The Left Image is a negative control without the prescence of ab3479. Image magnification is 60X.

    • Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)
      Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody - ChIP Grade (ab3479)

      Immunocytochemical immunoflurescence analysis of NIH-3T3 cells labelling mSin3A using ab3479. Formalin-fixed cells were permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were then incubated with ab3479 in 3% BSA-PBS at a dilution of 1:100 overnight in a 4°C high humidity environment. Cells were then washed with PBST and incubated with a green DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Cells were counterstained blue with DAPI or Hoechst labelling the nuclear DNA and red against actin using an Alexa Fluor® 554 conjugate. The Left Image is a negative control without the prescence of ab3479. Image magnification is 60X.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody - ChIP Grade (ab3479)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody - ChIP Grade (ab3479)
      ab3479 (2µg/ml) staining mSin3A in human duodenum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclei of epithelial cells.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1/ EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (36)

    ab3479 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3479 は 36 報の論文で使用されています。

    • Hwang I  et al. CIC is a critical regulator of neuronal differentiation. JCI Insight 5:N/A (2020). PubMed: 32229723
    • Pinto DO  et al. Effect of transcription inhibition and generation of suppressive viral non-coding RNAs. Retrovirology 16:13 (2019). PubMed: 31036006
    • Fuller NO  et al. CoREST Complex-Selective Histone Deacetylase Inhibitors Show Prosynaptic Effects and an Improved Safety Profile To Enable Treatment of Synaptopathies. ACS Chem Neurosci 10:1729-1743 (2019). PubMed: 30496686
    • Zhu J  et al. NKX2-8 deletion-induced reprogramming of fatty acid metabolism confers chemoresistance in epithelial ovarian cancer. EBioMedicine 43:238-252 (2019). PubMed: 31047858
    • Meier-Soelch J  et al. RNAi-Based Identification of Gene-Specific Nuclear Cofactor Networks Regulating Interleukin-1 Target Genes. Front Immunol 9:775 (2018). PubMed: 29755455
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-10 of 10 Abreviews or Q&A

    ChIP abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Mouse Tissue lysate - whole (Mouse brain postnatal day 1)
    Negative control
    Input genomic DNA processed in parallel to the mSIN3A ChIP sample and analyzed for target promoter enrichment.
    Specification
    Mouse brain postnatal day 1
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 0 second(s)
    Positive control
    Our mSIN3A ChIP pull down sample analyzed for target promoter enrichment.
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 23 2020

    Western blot abreview for Anti-mSin3A antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (Embryonic stem cell)
    Gel Running Conditions
    Non-reduced Denaturing (7%)
    Loading amount
    60 µg
    Specification
    Embryonic stem cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 19 2016

    Western blot abreview for Anti-mSin3A antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (7%gel)
    Sample
    Mouse Tissue lysate - whole (hippocampus)
    Specification
    hippocampus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 30 2015

    Immunoprecipitation abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein A/G
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Specification
    HEK293T
    Total protein in input
    40 µg
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 05 2013

    ChIP abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Detection step
    Semiquantitative PCR
    Sample
    Human Cell lysate - nuclear (MCF7)
    Specification
    MCF7
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: FORMALDEHYDE 10%
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 05 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate
    Sample
    Mouse Tissue sections (BREAST CANCER TISSUE)
    Specification
    BREAST CANCER TISSUE
    Permeabilization
    Yes - xylene
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 05 2013

    Western blot abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    1e+006 cells
    Gel Running Conditions
    Reduced Denaturing (8)
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Specification
    HEK293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 04 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (NIH/3T3)
    Specification
    NIH/3T3
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Mrs. Filomena Ricciardi

    Verified customer

    投稿 Jun 26 2012

    Western blot abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (THP-1 cells)
    Loading amount
    10 µg
    Specification
    THP-1 cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 21 2007

    Western blot abreview for Anti-mSin3A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (Lung)
    Loading amount
    10 µg
    Specification
    Lung
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

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    投稿 Aug 21 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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