HRP Anti-MSI2 抗体 [EP1305Y] (ab199252)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1305Y] to MSI2
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
製品の概要
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製品名
HRP Anti-MSI2 antibody [EP1305Y]
MSI2 一次抗体 製品一覧 -
製品の詳細
HRP Rabbit monoclonal [EP1305Y] to MSI2 -
由来種
Rabbit -
標識
HRP -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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エピトープ
Based on the immunogen sequence for this antibody, it is not predicted to detect the shorter isoforms of MSI2. -
ポジティブ・コントロール
- WB: SW480 whole cell lysate.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1305Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab199252の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Detects a band of approximately 39,37 kDa (predicted molecular weight: 35 kDa).
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特記事項 |
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WB
1/5000. Detects a band of approximately 39,37 kDa (predicted molecular weight: 35 kDa). |
ターゲット情報
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機能
RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system. -
組織特異性
Ubiquitous; detected at low levels. -
関連疾患
Note=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23). -
配列類似性
Belongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Cytoplasm. Associated with polysomes. - Information by UniProt
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参照データベース
- Entrez Gene: 124540 Human
- Entrez Gene: 76626 Mouse
- Entrez Gene: 360596 Rat
- Omim: 607897 Human
- SwissProt: Q96DH6 Human
- SwissProt: Q920Q6 Mouse
- Unigene: 658922 Human
- Unigene: 400451 Mouse
see all -
別名
- FLJ36569 antibody
- MGC3245 antibody
- Msi2 antibody
see all
画像
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HRP Anti-MSI2 antibody [EP1305Y] (ab199252) at 1/5000 dilution + SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 37,39 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199252 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab199252 は論文での使用が確認できていません。