Anti-MS2 抗体 (ab236949)
Key features and details
- Rabbit polyclonal to MS2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MS2 antibody
MS2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MS2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human MS2 aa 650-850.
Database link: P78325 -
ポジティブ・コントロール
- WB: Raji, THP-1 and Raw 264.7 whole cell lysates. IHC-P: Human lymph node and pancreas tissues. ICC/IF: MCF7 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purity >95%. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236949の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/200 - 1/500.
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WB |
1/500 - 1/5000. Predicted molecular weight: 89 kDa.
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ICC/IF |
1/50 - 1/200.
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特記事項 |
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IHC-P
1/200 - 1/500. |
WB
1/500 - 1/5000. Predicted molecular weight: 89 kDa. |
ICC/IF
1/50 - 1/200. |
ターゲット情報
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機能
Possible involvement in extravasation of leukocytes. -
組織特異性
Expressed on neutrophils and monocytes. -
配列類似性
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 101 Human
- Entrez Gene: 11501 Mouse
- Omim: 602267 Human
- SwissProt: P78325 Human
- SwissProt: Q05910 Mouse
- Unigene: 501574 Human
- Unigene: 15969 Mouse
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別名
- A Disintegrin And Metalloproteinase domain 8 antibody
- ADAM 8 antibody
- ADAM 8 precursor antibody
see all
画像
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All lanes : Anti-MS2 antibody (ab236949) at 1/500 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 89 kDa -
Paraffin-embedded human lymph node tissue stained for MS2 using ab236949 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Paraffin-embedded human pancreas tissue stained for MS2 using ab236949 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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MCF7 (human breast adenocarcinoma cell line) cells stained for MS2 (green) using ab236949 at 1/133 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI (Blue).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab236949 は論文での使用が確認できていません。