Anti-MRP3 抗体 [M3II-9] (ab3375)

Reviews (3)Q&A (2)References (9)
Western blot - Anti-MRP3 antibody [M3II-9] (ab3375)

    Key features and details

    • Mouse monoclonal [M3II-9] to MRP3
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-MRP3 antibody [M3II-9]
      MRP3 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [M3II-9] to MRP3

    • 由来種

      Mouse

    • 特異性

      This antibody does not cross react with MDR1, MRP1, MRP2 or MRP5 gene products

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Mouse, Human

    • 免疫原

      Fusion protein:

      SEMGPYPALL QRNGSFANFL CNYAPDEDQG HLEDSWTALE GAEDKEALLI EDTLSNHTD LTDNDPVTYV VQKQFMRQLS ALSSDGEGQG RPVPRRHLGPS EKVQVTEAKA DGALTQEEKA

      , corresponding to amino acids 830-949 of Human MRP3.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • エピトープ

      This antibody detects with an internal epitope of the membrane transport protein of MRP3

    • ポジティブ･コントロール

      • Cell lines: Drug sensitive parental cell lines and their multidrug resistant derivatives

    • 特記事項

      The rat species was added as a result of positive Abreviews and publication PMID 22112382. In response to few recent complaints, we have decided to remove this species and no longer guarantee it. Please contact technical@abcam.com for any inquiry.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab3375 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB 1/20 - 1/50. Detects a band of approximately 190 kDa.

    ターゲット情報

    • 機能

      May act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes.

    • 組織特異性

      Mainly expressed in the liver. Also expressed in small intestine, colon, prostate, testis, brain and at a lower level in the kidney.

    • 配列類似性

      Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • ABC 31 antibody
        • ABC31 antibody
        • ABCC 3 antibody
        • ABCC3 antibody
        • ATP binding cassette sub family C (CFTR/MRP) member 3 antibody
        • ATP binding cassette sub family C member 3 antibody
        • ATP-binding cassette sub-family C member 3 antibody
        • Canalicular multispecific organic anion transporter 2 antibody
        • Canicular multispecific organic anion transporter antibody
        • CMOAT 2 antibody
        • CMOAT2 antibody
        • EST90757 antibody
        • MLP 2 antibody
        • MLP2 antibody
        • MOAT D antibody
        • MOAT-D antibody
        • MOATD antibody
        • MRP 3 antibody
        • MRP3_HUMAN antibody
        • Multi specific organic anion transporter D antibody
        • Multi-specific organic anion transporter D antibody
        • Multidrug resistance associated protein 3 antibody
        • Multidrug resistance associated protein antibody
        • Multidrug resistance-associated protein 3 antibody
        • Multispecific organic anion tranporter D antibody
        see all

      画像

      • Western blot - Anti-MRP3 antibody [M3II-9] (ab3375)
        Western blot - Anti-MRP3 antibody [M3II-9] (ab3375)

         HepG2 cells were incubated at 37°C for 24h with vehicle control (0 μM) and different concentrations of clotrimazole (ab120744) in DMSO. Increased expression of MRP3 (ab3375) correlates with an increase in clotrimazole concentration, as described in literature.

        Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3375 at 1/50 dilution and ab9484 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.

      参考文献 (9)

      ab3375 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3375 は 9 報の論文で使用されています。

      • Wang H  et al. Effects of histone deacetylase inhibitors on ATP-binding cassette transporters in lung cancer A549 and colorectal cancer HCT116 cells. Oncol Lett 18:63-71 (2019). PubMed: 31289473
      • Han SH  et al. Prognostic implication of ABC transporters and cancer stem cell markers in patients with stage III colon cancer receiving adjuvant FOLFOX-4 chemotherapy. Oncol Lett 17:5572-5580 (2019). PubMed: 31186779
      • Madden LR  et al. Bioprinted 3D Primary Human Intestinal Tissues Model Aspects of Native Physiology and ADME/Tox Functions. iScience 2:156-167 (2018). PubMed: 30428372
      • Zukauskas A  et al. Transporters MRP1 and MRP2 Regulate Opposing Inflammatory Signals To Control Transepithelial Neutrophil Migration during Streptococcus pneumoniae Lung Infection. mSphere 3:N/A (2018). PubMed: 29976647
      • Canet MJ  et al. Altered regulation of hepatic efflux transporters disrupts acetaminophen disposition in pediatric nonalcoholic steatohepatitis. Drug Metab Dispos 43:829-35 (2015). PubMed: 25788542
      • Ding L  et al. Danning tablets attenuates a-naphthylisothiocyanate-induced cholestasis by modulating the expression of transporters and metabolic enzymes. BMC Complement Altern Med 14:249 (2014). WB . PubMed: 25033983
      • Hardwick RN  et al. Increased Susceptibility to Methotrexate-Induced Toxicity in Nonalcoholic Steatohepatitis. Toxicol Sci : (2014). PubMed: 25080921
      • Hardwick RN  et al. Molecular mechanism of altered ezetimibe disposition in nonalcoholic steatohepatitis. Drug Metab Dispos 40:450-60 (2012). WB ; Rat . PubMed: 22112382
      • Hardwick RN  et al. Variations in ATP-binding cassette transporter regulation during the progression of human nonalcoholic fatty liver disease. Drug Metab Dispos 39:2395-402 (2011). WB, IHC-P ; Human . PubMed: 21878559

      レビューと Q&A

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      1-5 of 5 Abreviews or Q&A

      Question

      To technical support,



      I would like to order a complete set of antibodies and controls from Abcam for WB analysis. Could you check if these antibodies would suit for the analysis. In addition, could you recommend some positive controls.







      https://www.abcam.com/P-Glycoprotein-antibody-JSB-1-ab3366.html



      https://www.abcam.com/P-Glycoprotein-antibody-JSB-1-ab3366.html





      https://www.abcam.com/MRP2-antibody-M2-III-6-ab3373.html



      https://www.abcam.com/MRP2-antibody-M2-III-6-ab3373.html





      https://www.abcam.com/MRP3-antibody-M3II-9-ab3375.html



      https://www.abcam.com/MRP3-antibody-M3II-9-ab3375.html





      https://www.abcam.com/MRP1-antibody-MRPm5-ab24102.html



      https://www.abcam.com/MRP1-antibody-MRPm5-ab24102.html





      https://www.abcam.com/BCRPABCG2-antibody-ab63907.html



      https://www.abcam.com/BCRPABCG2-antibody-ab63907.html





      https://www.abcam.com/MRP4-antibody-ab77184.html



      https://www.abcam.com/MRP4-antibody-ab77184.html





      https://www.abcam.com/Goat-Mouse-IgG-HL-Alkaline-Phosphatase-ab97020.html



      https://www.abcam.com/Goat-Mouse-IgG-HL-Alkaline-Phosphatase-ab97020.html





      https://www.abcam.com/Goat-Rabbit-IgG-HL-Alkaline-Phosphatase-ab97048.html



      https://www.abcam.com/Goat-Rabbit-IgG-HL-Alkaline-Phosphatase-ab97048.html





      https://www.abcam.com/Rabbit-Goat-IgG-HL-Alkaline-Phosphatase-ab97097.html



      https://www.abcam.com/Rabbit-Goat-IgG-HL-Alkaline-Phosphatase-ab97097.html





      https://www.abcam.com/NBT--BCIP-Solution-Ready-to-Use-Alkaline-Phosphatase-chromogen-ab7468.html



      https://www.abcam.com/NBT--BCIP-Solution-Ready-to-Use-Alkaline-Phosphatase-chromogen-ab7468.html





      https://www.abcam.com/alpha-Tubulin-antibody-DM1A-Loading-Control-ab7291.html



      https://www.abcam.com/alpha-Tubulin-antibody-DM1A-Loading-Control-ab7291.html







      With kind regards,

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      Answer

      Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
      I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab24102.
      https://www.abcam.com/Human-liver-tissue-lysate-total-protein-ab29889.html https://www.abcam.com/Human-liver-tissue-lysate-total-protein-ab29889.html.
      MRP4 is barely detectable in liver and therefore I recommend to use ab30304 (Prostate (Human) Tissue Lysate - adult normal tissue) as positive control.
      https://www.abcam.com/Human-prostate-tissue-lysate-total-protein-ab30304.html https://www.abcam.com/Human-prostate-tissue-lysate-total-protein-ab30304.html.
      With ab63907 we used ab29745 (Placenta (Human) Tissue Lysate - adult normal tissue) successfully as control.
      https://www.abcam.com/Human-placenta-tissue-lysate-total-protein-ab29745.html https://www.abcam.com/Human-placenta-tissue-lysate-total-protein-ab29745.html.
      I hope this information is helpful and wish you good luck with your research.

      Read More

      Western blot abreview for Anti-MRP3 antibody [M3II-9]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Tissue lysate - whole (Liver)
      Loading amount
      80 µg
      Specification
      Liver
      Gel Running Conditions
      Non-reduced Denaturing (10%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
      Read More

      Abcam user community

      Verified customer

      投稿 Aug 06 2010

      Western blot abreview for Anti-MRP3 antibody [M3II-9]

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Liver homogenate)
      Loading amount
      25 µg
      Specification
      Liver homogenate
      Gel Running Conditions
      Reduced Denaturing (7.5%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      投稿 Sep 17 2009

      Western blot abreview for Anti-MRP3 antibody [M3II-9]

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - other (lung carcinoma)
      Loading amount
      25 µg
      Specification
      lung carcinoma
      Gel Running Conditions
      Reduced Denaturing (7.5%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      投稿 Aug 19 2009

      Question

      I am interested in your antibodies for BCRP (ab3380: Clone BXP-21), MDR1 (ab10333: Clone 4E3), MRP2 (ab3373: Clone M2 III-6) and MRP3 (ab3375: Clone M3II-9). As I will be using these antibodies for FACS, I would like to know whether you have any of these in a conjugated version (FITC or PE) as well? If not, do you have any matching secondairy antibodies which are conjugated with FITC or PE? Are these antibodies available at the moment? If I buy one vial of each of these antibodies in one go, will there be any special price offer? I would prefer to detect only the proteins which are present in the plasma membrane. As the MRP antibodies are reacting with an internal epitope, it will be necessary to permeabilize the plasma membrane. Do you know of any more or less selective technique to permeabilize the plasma membrane resulting in a selective staining of MRP2 and MRP3 in the plasma membrane only? I am lokoing forward to your reply. Thanks for your help!

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      Answer

      Thank you for your enquiry and for your patience. Unfortunately, none of these antibodies are available in a conjugated form but we do have a wide range of secondary antibodies to choose from (including ones conjugated to FITC and PE). At the bottom of the online datasheets for each of these primary antibodies, there is a list of compatible secondary antibodies that can be used. We unfortunately can't offer any discounts. All the information that we have regarding these antibodies is located on the online datasheets, and ab10333 and ab3373 have been tested for application in FACS but ab3380 and ab3375 have not to our knowledge. If you have any more questions, please contact us again.

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