Key features and details
- Mouse monoclonal [M2 III-6] to MRP2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
製品名Anti-MRP2 antibody [M2 III-6]
MRP2 一次抗体 製品一覧
製品の詳細Mouse monoclonal [M2 III-6] to MRP2
特異性This antibody detects MRP 2. It does not cross-react with human MDR 1, MRP 1, MRP 3 or MRP 5 gene products.
アプリケーション適用あり: WBmore details
Fusion protein corresponding to Human MRP2 aa 1339-1541 (C terminal).
エピトープThis antibody reacts with an internal epitope of MRP 2.
We received mixed feedback about rat species so we are removing this from tested species list for now. The immunogen is 84% homologous to human protein so theoretically it should cross react however considering the latest customer feedback we no longer guarantee this species.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: 0.7% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab3373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/20 - 1/50. Predicted molecular weight: 174 kDa.
See Scheffer GL et al. 2000.
The samples MUST NOT be boiled before running the gel as multi-pass transmembrane proteins, like MRP2, aggregate severely at high temperatures, which increases as a direct function of temperature. We recommend heating the samples at 60-70C for 15-20 minutes or at 36C for 20 minutes as mentioned in PLoS Pathog 9:e1003244 (2013).
機能Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
組織特異性Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.
関連疾患Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.
配列類似性Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
- Information by UniProt
- ABC30 antibody
- abcC2 antibody
- ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
All lanes : Anti-MRP2 antibody [M2 III-6] (ab3373) at 1/20 dilution
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : ABCC2 knockout A549 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 174 kDa
Lanes 1 - 3: Merged signal (red and green). Green - ab3373 observed at 210 kDa. Red - loading control, ab52866, observed at 50 kDa.
ab3373 was shown to recognize ABCC2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab3373 and ab52866 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/20 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Anti-MRP2 antibody [M2 III-6] (ab3373) at 1/500 dilution + WIF-B cells, the hepatoma-derived hybrid cell line at 30 µg with 5% milk in PBST
HRP conjugated Donkey anti-Mouse at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 174 kDa
Observed band size: 130-250 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
ab3373 は 62 報の論文で使用されています。
- Subramani PA et al. Plasmodium vivax liver stage assay platforms using Indian clinical isolates. Malar J 19:214 (2020). PubMed: 32571333
- Zhang Y et al. Cx32 mediates cisplatin resistance in human ovarian cancer cells by affecting drug efflux transporter expression and activating the EGFR-Akt pathway. Mol Med Rep 19:2287-2296 (2019). PubMed: 30664215
- Hiemstra S et al. High-throughput confocal imaging of differentiated 3D liver-like spheroid cellular stress response reporters for identification of drug-induced liver injury liability. Arch Toxicol 93:2895-2911 (2019). PubMed: 31552476
- Balasubramaniyan N et al. Up-regulation of miR-let7a-5p Leads to Decreased Expression of ABCC2 in Obstructive Cholestasis. Hepatol Commun 3:1674-1686 (2019). PubMed: 31832574
- Katsuda T et al. Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes. Elife 8:N/A (2019). PubMed: 31393263