製品の概要

  • 製品名
    Anti-MRP1 antibody [MRPr1]
    MRP1 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [MRPr1] to MRP1
  • 由来種
    Rat
  • 特異性
    This antibody detects MRP 1. It does not cross-react with the human MDR 1 and MDR 3 P glycoprotein gene products.
  • アプリケーション
    適用あり: IHC-P, WB, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Bacterial fusion protein of human MRP 1 containing a segment of 168 amino acids inthe amino-proximal half of the protein.

  • エピトープ
    Internal epitope.
  • ポジティブ･コントロール
    • Cell Lines: Drug-sensitive parental cell lines and their multidrug-resistant derivatives. Tissue section: Breast carcinoma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr 1/20 - 1/50.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
  • 組織特異性
    Lung, testis and peripheral blood mononuclear cells.
  • 配列類似性
    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • ABC 29 antibody
      • ABC29 antibody
      • ABCC 1 antibody
      • ABCC antibody
      • Abcc1 antibody
      • ATP binding cassette sub family C (CFTR/MRP) member 1 antibody
      • ATP binding cassette sub-family C member 1 antibody
      • ATP binding cassette subfamily C member 1 antibody
      • ATP binding cassette transporter variant ABCC1delta ex13 antibody
      • ATP binding cassette transporter variant ABCC1delta ex13&14 antibody
      • ATP binding cassette transporter variant ABCC1delta ex25 antibody
      • ATP binding cassette transporter variant ABCC1delta ex25&26 antibody
      • ATP binding cassette, sub-family C (CFTR/MRP), member 1 antibody
      • ATP-binding cassette sub-family C member 1 antibody
      • DKFZp686N04233 antibody
      • DKFZp781G125 antibody
      • GS X antibody
      • GSX antibody
      • Leukotriene C(4) transporter antibody
      • LTC4 transporter antibody
      • MRP 1 antibody
      • MRP antibody
      • MRP1 antibody
      • MRP1_HUMAN antibody
      • Multidrug resistance associated protein 1 antibody
      • Multidrug resistance protein antibody
      • Multidrug resistance-associated protein 1 antibody
      • Multiple drug resistance associated protein antibody
      • Multiple drug resistance protein 1 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-MRP1 antibody [MRPr1] (ab3368)
      Immunocytochemistry/ Immunofluorescence - Anti-MRP1 antibody [MRPr1] (ab3368)
      ICC/IF image of ab3368 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3368,5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [MRPr1] (ab3368)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [MRPr1] (ab3368)
      IHC image of ab3368 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3368, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    参考文献

    This product has been referenced in:
    • Fan H  et al. Knockdown of long non-coding RNA PVT1 reverses multidrug resistance in colorectal cancer cells. Mol Med Rep 17:8309-8315 (2018). Read more (PubMed: 29693171) »
    • Pan X  et al. Downregulation of eIF4G by microRNA-503 enhances drug sensitivity of MCF-7/ADR cells through suppressing the expression of ABC transport proteins. Oncol Lett 13:4785-4793 (2017). WB . Read more (PubMed: 28599480) »

    See all 7 Publications for this product

    ab3368 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    2 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-MRP1 antibody [MRPr1]

     Below Average
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - other (MCF7)
    Loading amount
    30 µg
    Specification
    MCF7
    Treatment
    drug or DMSO
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (lysates not heated and no b-mercapto or DTT added to loading buffer)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Read More
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    Abcam user community

    Verified customer

    投稿 Apr 05 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MRP1 antibody [MRPr1]

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human Breast)
    Specification
    Human Breast
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Blocking step
    No blocking step used for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Read More
    Username

    Abcam user community

    Verified customer

    投稿 Apr 05 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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