Anti-MRP1 抗体 [MRPr1] (ab3368)

Reviews (2) Submit a question References (14)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [MRPr1] (ab3368)
  • Immunocytochemistry/ Immunofluorescence - Anti-MRP1 antibody [MRPr1] (ab3368)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [MRPr1] (ab3368)

Key features and details

  • Rat monoclonal [MRPr1] to MRP1
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-MRP1 antibody [MRPr1]
    MRP1 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [MRPr1] to MRP1

  • 由来種

    Rat

  • 特異性

    This antibody detects MRP 1. It does not cross-react with the human MDR 1 and MDR 3 P glycoprotein gene products.

  • アプリケーション

    適用あり: IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Bacterial fusion protein of human MRP 1 containing a segment of 168 amino acids inthe amino-proximal half of the protein.

  • エピトープ

    Internal epitope.

  • ポジティブ･コントロール

    • Cell Lines: Drug-sensitive parental cell lines and their multidrug-resistant derivatives. Tissue section: Breast carcinoma, liver.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.

  • 組織特異性

    Lung, testis and peripheral blood mononuclear cells.

  • 配列類似性

    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ABC 29 antibody
      • ABC29 antibody
      • ABCC 1 antibody
      • ABCC antibody
      • Abcc1 antibody
      • ATP binding cassette sub family C (CFTR/MRP) member 1 antibody
      • ATP binding cassette sub-family C member 1 antibody
      • ATP binding cassette subfamily C member 1 antibody
      • ATP binding cassette transporter variant ABCC1delta ex13 antibody
      • ATP binding cassette transporter variant ABCC1delta ex13&14 antibody
      • ATP binding cassette transporter variant ABCC1delta ex25 antibody
      • ATP binding cassette transporter variant ABCC1delta ex25&26 antibody
      • ATP binding cassette, sub-family C (CFTR/MRP), member 1 antibody
      • ATP-binding cassette sub-family C member 1 antibody
      • DKFZp686N04233 antibody
      • DKFZp781G125 antibody
      • GS X antibody
      • GSX antibody
      • Leukotriene C(4) transporter antibody
      • LTC4 transporter antibody
      • MRP 1 antibody
      • MRP antibody
      • MRP1 antibody
      • MRP1_HUMAN antibody
      • Multidrug resistance associated protein 1 antibody
      • Multidrug resistance protein antibody
      • Multidrug resistance-associated protein 1 antibody
      • Multiple drug resistance associated protein antibody
      • Multiple drug resistance protein 1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [MRPr1] (ab3368)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [MRPr1] (ab3368)

      IHC staining of ab3368 on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1/20 dilution of the primary antibody for 60 minutes at room temperature. After incubation with biotinylated anti-rat antibody, the tissue was labeled with BioLegend's HRP labeling reagent followed by addition of DAB chromogen for detection and hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar 50 µm

    • Immunocytochemistry/ Immunofluorescence - Anti-MRP1 antibody [MRPr1] (ab3368)
      Immunocytochemistry/ Immunofluorescence - Anti-MRP1 antibody [MRPr1] (ab3368)

      ICC/IF image of ab3368 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3368,5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [MRPr1] (ab3368)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [MRPr1] (ab3368)
      IHC image of ab3368 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3368, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    参考文献 (14)

    ab3368 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3368 は 14 報の論文で使用されています。

    • Wu J  et al. Long noncoding RNA UCA1 targets miR-582-5p and contributes to the progression and drug resistance of bladder cancer cells through ATG7-mediated autophagy inhibition. Onco Targets Ther 12:495-508 (2019). PubMed: 30666128
    • Cao JY  et al. A Genome-wide Haploid Genetic Screen Identifies Regulators of Glutathione Abundance and Ferroptosis Sensitivity. Cell Rep 26:1544-1556.e8 (2019). PubMed: 30726737
    • Krohn M  et al. Generation and Characterization of an Abcc1 Humanized Mouse Model (hABCC1flx/flx ) with Knockout Capability. Mol Pharmacol 96:138-147 (2019). PubMed: 31189668
    • Yao L & Zhang Z The reversal of MRP1 expression induced by low-frequency and low-intensity ultrasound and curcumin mediated by VEGF in brain glioma. Onco Targets Ther 12:3581-3593 (2019). PubMed: 31190861
    • Hellinen L  et al. Retinal Pigment Epithelial Cell Line with Fast Differentiation and Improved Barrier Properties. Pharmaceutics 11:N/A (2019). PubMed: 31412689
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-MRP1 antibody [MRPr1]

     Below Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (MCF7)
    Loading amount
    30 µg
    Specification
    MCF7
    Treatment
    drug or DMSO
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (lysates not heated and no b-mercapto or DTT added to loading buffer)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 05 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MRP1 antibody [MRPr1]

     Average
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human Breast)
    Specification
    Human Breast
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Blocking step
    No blocking step used for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 05 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com