Application
Western blot
Sample
Pig Cell lysate - whole cell (Testis)
Gel Running Conditions
Reduced Non-Denaturing (Native) (8%)
Loading amount
40 µg
Specification
Testis
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 23°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: Goat anti mouse
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Detection
Detection method
Odyssey IR
Negative control
Non-specific at 50 kDa
Exposure
2 minute(s) and 0 second(s)
Positive control
Non-specific at 70 and 50 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dorothy Elsken
Verified customer
投稿 Jul 22 2019