Application
Western blot
Sample
Rat Cell lysate - whole cell (Liver)
Gel Running Conditions
Reduced Denaturing (Samples were not denatured prior to gel loading)
Loading amount
80 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in PBST
Dilution
1/5000
Secondary antibody
Dilution
1/40000
Name
Non-Abcam antibody was used: Goat anti- mouse Santa Cruz sc-2005
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Regular ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 190 kDa Non-specific: ~160 kDa
Positive control
normal human liver whole cell lysate
Additional data
Additional Notes
A 160 kDa band is only visible at extended exposure, and is not shown on the image provided.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 14 2011