Anti-Mre11 抗体 - ChIP Grade (ab12159)
Key features and details
- Rabbit polyclonal to Mre11 - ChIP Grade
- Suitable for: ChIP
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Mre11 antibody - ChIP Grade
Mre11 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Mre11 - ChIP Grade -
由来種
Rabbit -
アプリケーション
適用あり: ChIPmore details -
種交差性
交差種: Saccharomyces cerevisiae -
免疫原
Synthetic peptide:
C-SQAESSKPTSKPK
conjugated to KLH, corresponding to amino acids 578-592 of S. cerevisiae Mre11. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12159の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ChIP |
Use at an assay dependent dilution.
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| 特記事項 |
|---|
|
ChIP
Use at an assay dependent dilution. |
ターゲット情報
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機能
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. -
関連疾患
Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course. -
配列類似性
Belongs to the MRE11/RAD32 family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents. - Information by UniProt
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別名
- AT like disease antibody
- Ataxia telangiectasia disorder like antibody
- ATLD antibody
see all
画像
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ChIP - Anti-Mre11 antibody - ChIP Grade (ab12159)
A Saccharomyces cerevisiae strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin.
Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR Green (Invitrogen), and detected using a Fuji scanning fluorometer.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab12159 は 4 報の論文で使用されています。
- Li Y et al. The DNA Repair Nuclease MRE11A Functions as a Mitochondrial Protector and Prevents T Cell Pyroptosis and Tissue Inflammation. Cell Metab 30:477-492.e6 (2019). PubMed: 31327667
- Lu X et al. GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival. Nucleic Acids Res 47:10977-10993 (2019). PubMed: 31612207
- Li Z et al. Destabilization of linker histone H1.2 is essential for ATM activation and DNA damage repair. Cell Res 28:756-770 (2018). PubMed: 29844578
- Baek IJ et al. The mre11 A470 alleles influence the hereditability and the segregation of telosomes in Saccharomyces cerevisiae. PLoS One 12:e0183549 (2017). PubMed: 28886051
