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    mre11-antibody-12d7-bsa-and-azide-free-ab214.pdf

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Anti-Mre11 抗体 [12D7] - BSA and Azide free (ab214)

  • Datasheet
  • SDS
Reviews (10) Submit a question References (79)

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Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
  • Flow Cytometry - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)

Key features and details

  • Mouse monoclonal [12D7] to Mre11 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-Fr, IHC-P, IP, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human Mre11 protein (ab114277)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
一次抗体
Product image
Anti-RPA32/RPA2 antibody [EPR2877Y] (ab76420)

関連製品

製品の概要

  • 製品名

    Anti-Mre11 antibody [12D7] - BSA and Azide free
    Mre11 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [12D7] to Mre11 - BSA and Azide free
  • 由来種

    Mouse
  • アプリケーション

    適用あり: Flow Cyt, IHC-Fr, IHC-P, IP, ICC/IF, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Mre11 aa 150-600.

  • ポジティブ・コントロール

    • WB HEK-293T, A431, HeLa, HepG2, PC-12 whole cell lysate; ICC: HeLa cells.
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 10th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Constituent: 100% PBS
  • キャリア・フリー

    はい
  • Concentration information loading...
  • 精製度

    Tissue culture supernatant
  • ポリ/モノ

    モノクローナル
  • クローン名

    12D7
  • ミエローマ

    NS1
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    kappa
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Telomeres
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Non Homol. End Joining
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Mre11 protein (ab114277)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab214の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr (1)
Use at an assay dependent concentration.
IHC-P (1)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

For normal lymphoblastoid cell lines.

ICC/IF (2)
1/100 - 1/1000.
WB (4)
1/500 - 1/3000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).

(see Robinson et al).

特記事項
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

For normal lymphoblastoid cell lines.

ICC/IF
1/100 - 1/1000.
WB
1/500 - 1/3000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).

(see Robinson et al).

ターゲット情報

  • 機能

    Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • 関連疾患

    Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
  • 配列類似性

    Belongs to the MRE11/RAD32 family.
  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在

    Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Target information above from: UniProt accession P49959 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 4361 Human
    • Entrez Gene: 17535 Mouse
    • Entrez Gene: 64046 Rat
    • Omim: 600814 Human
    • SwissProt: P49959 Human
    • SwissProt: Q61216 Mouse
    • SwissProt: Q9JIM0 Rat
    • Unigene: 192649 Human
    • Unigene: 149071 Mouse
    • Unigene: 209040 Rat
    see all
  • 別名

    • AT like disease antibody
    • Ataxia telangiectasia disorder like antibody
    • ATLD antibody
    • DNA recombination and repair protein antibody
    • Double strand break repair protein MRE11A antibody
    • Double-strand break repair protein MRE11A antibody
    • endo/exonuclease Mre11 antibody
    • HNGS1 antibody
    • meiotic recombination (S. cerevisiae) 11 homolog A antibody
    • Meiotic recombination 11 homolog 1 antibody
    • meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • Meiotic recombination 11 homolog A antibody
    • MmMRE11A antibody
    • Mre 11 antibody
    • MRE 11a antibody
    • MRE 11b antibody
    • MRE11 homolog 1 antibody
    • MRE11 homolog A antibody
    • MRE11 homolog double strand break repair nuclease antibody
    • MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • MRE11 meiotic recombination 11 homolog A antibody
    • MRE11_HUMAN antibody
    • MRE11A antibody
    • MRE11b antibody
    • OTTHUMP00000236830 antibody
    • OTTHUMP00000236831 antibody
    • OTTHUMP00000236832 antibody
    • OTTHUMP00000236833 antibody
    see all

画像

  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    All lanes : Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : anti-mouse IgG HRP-conjugated antibody

    Predicted band size: 79 kDa



    7.5% SDS-PAGE

  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)

    Immunocytochemical analysis of, 4% paraformaldehyde-fixed at RT for 15 min, HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mre-11 (green) with ab214 at 1/200 dilution. Blue: Hoechst 33342 staining. Scale bar= 10 μm.

  • Flow Cytometry - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Flow Cytometry - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)

    Overlay histogram showing HeLa cells stained with ab214 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab214, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214) at 1/500 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

    Secondary
    Anti-mouse IgG HRP-conjugated antibody

    Developed using the ECL technique.

    Predicted band size: 79 kDa



    7.5% SDS-PAGE

    The signal was developed with Trident ECL plus-Enhanced.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    All lanes : Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : Human Mre-11-transfected HEK-293T whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : anti-mouse IgG HRP-conjugated antibody

    Predicted band size: 79 kDa



    This image was generated using the ascites version of the product.

    7.5% SDS-PAGE

  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)Image supplied by Dr Domenico Delia, Istituto Nazionale Tumori, Italy.

    Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells.
    (Panel D - negative control).

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)
    Western blot - Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214)This image is courtesy of an anonymous Abreview
    All lanes : Anti-Mre11 antibody [12D7] - BSA and Azide free (ab214) at 1/500 dilution

    Lane 1 : Mouse breast cancer cell line - whole cell lysate. Transfected with vector control.
    Lane 2 : Mouse breast cancer cell line - whole cell lysate. Transfected with knockdown shRNA targeting Mre11 gene.

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated Goat anti-mouse IgG polyclonal at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 79 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This image was generated using the ascites version of the product.

    See Abreview

プロトコール

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (79)

ab214 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab214 は 79 報の論文で使用されています。

  • Elkholi IE  et al. Investigating the causal role of MRE11A p.E506* in breast and ovarian cancer. Sci Rep 11:2409 (2021). PubMed: 33510186
  • Na J  et al. SPRTN protease-cleaved MRE11 decreases DNA repair and radiosensitises cancer cells. Cell Death Dis 12:165 (2021). PubMed: 33558481
  • Hu S  et al. LINCS gene expression signature analysis revealed bosutinib as a radiosensitizer of breast cancer cells by targeting eIF4G1. Int J Mol Med 47:N/A (2021). PubMed: 33693953
  • Kang JW  et al. PUMA facilitates EMI1-promoted cytoplasmic Rad51 ubiquitination and inhibits DNA repair in stem and progenitor cells. Signal Transduct Target Ther 6:129 (2021). PubMed: 33785736
  • Kilgas S  et al. p97/VCP inhibition causes excessive MRE11-dependent DNA end resection promoting cell killing after ionizing radiation. Cell Rep 35:109153 (2021). PubMed: 34038735
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 10 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Mre11 antibody [12D7]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (LLC-PK1 cell line)
Permeabilization
Yes - 0.5% Triton-X 100
Specification
LLC-PK1 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: R.T°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 10 2016

Immunohistochemistry (Frozen sections) abreview for Anti-Mre11 [12D7] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Human Tissue sections (Normal or gastric cancer tissues)
Specification
Normal or gastric cancer tissues
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 20 2014

Immunocytochemistry abreview for Anti-Mre11 [12D7] antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Sample
Mouse Cultured Cells (Mouse embryonic fibroblasts)
Specification
Mouse embryonic fibroblasts
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 19 2014

Immunocytochemistry abreview for Anti-Mre11 [12D7] antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Human Cultured Cells (Hela cell line)
Specification
Hela cell line
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 18 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Mre11 [12D7] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Sample
Human Cell (Human colon cancer cell lines)
Specification
Human colon cancer cell lines
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 15 2013

Western blot abreview for Anti-Mre11 antibody [12D7]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (7.5)
Sample
Mouse Cell lysate - whole cell (Mouse breast cancer cell line)
Specification
Mouse breast cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 15 2013

Western blot abreview for Anti-Mre11 antibody [12D7]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount
15 µg
Specification
Mouse embryonic fibroblasts
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 08 2011

Western blot abreview for Anti-Mre11 antibody [12D7]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - nuclear (human cancer cells (colon, bladder))
Loading amount
20 µg
Specification
human cancer cells (colon, bladder)
Gel Running Conditions
Reduced Denaturing (4-12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 12 2011

Western blot abreview for Anti-Mre11 antibody [12D7]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
20 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 17 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mre11 antibody [12D7]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Normal Bronchus)
Specification
Normal Bronchus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jun 15 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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製品・研究分野別
  • 癌
  • 循環器
  • 細胞生物学
  • 発生
  • エピジェネティクス&核内情報
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