Application
Western blot
Sample
Human Cell lysate - nuclear (human cancer cells (colon, bladder))
Loading amount
20 µg
Specification
human cancer cells (colon, bladder)
Gel Running Conditions
Reduced Denaturing (4-12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 19°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse IgG P0447 Dako Cytomation
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECLplus
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 79 kDa
Additional data
Additional Notes
Lane 1: Marker All Blue Biorad
Lane 2: nuclear extract A
Lane 3: nuclear extract B
Antibody works well with 1:500-1:1000 dilutions
Lane 2: nuclear extract A
Lane 3: nuclear extract B
Antibody works well with 1:500-1:1000 dilutions
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 12 2011