Anti-Mre11 抗体 [12D7] (ab214)
Key features and details
- Mouse monoclonal [12D7] to Mre11
- Suitable for: Flow Cyt, ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Mre11 antibody [12D7]
Mre11 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [12D7] to Mre11 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, ICC/IF, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Amino acids 182-582 of Mre11 expressed in E. coli.
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ポジティブ･コントロール
- WB HEK-293T, A431, HeLa, HepG2, PC-12 whole cell lysate; ICC: HeLa cells.
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特記事項
This product was changed from ascites to tissue culture supernatant on 10th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
12D7 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). (see Robinson et al). |
ターゲット情報
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機能
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. -
関連疾患
Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course. -
配列類似性
Belongs to the MRE11/RAD32 family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents. - Information by UniProt
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参照データベース
- Entrez Gene: 4361 Human
- Entrez Gene: 64046 Rat
- Omim: 600814 Human
- SwissProt: P49959 Human
- SwissProt: Q9JIM0 Rat
- Unigene: 192649 Human
- Unigene: 209040 Rat
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別名
- AT like disease antibody
- Ataxia telangiectasia disorder like antibody
- ATLD antibody
see all
画像
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![Western blot - Anti-Mre11 antibody [12D7] (ab214)](https://www.abcam.co.jp/ps/products/0/ab214/Images/ab214-397811-anti-mre11-antibody-wb-a431-hela-hek293t-hepg2-cells.jpg)
Western blot - Anti-Mre11 antibody [12D7] (ab214)All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : anti-mouse IgG HRP-conjugated antibody
Predicted band size: 79 kDa7.5% SDS-PAGE
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![Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)](https://www.abcam.co.jp/ps/products/0/ab214/Images/ab214-397810-anti-mre11-antibody-icc-hela-cells.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)Immunocytochemical analysis of, 4% paraformaldehyde-fixed at RT for 15 min, HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mre-11 (green) with ab214 at 1/200 dilution. Blue: Hoechst 33342 staining. Scale bar= 10 μm.
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![Flow Cytometry - Anti-Mre11 antibody [12D7] (ab214)](https://www.abcam.co.jp/ps/products/0/ab214/Images/ab214-3429-anti-mre11-antibody-12d7-flow-cytometry.jpg)
Flow Cytometry - Anti-Mre11 antibody [12D7] (ab214)Overlay histogram showing HeLa cells stained with ab214 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab214, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Mre11 antibody [12D7] (ab214)](https://www.abcam.co.jp/ps/products/0/ab214/Images/ab214-278591-anti-mre11-antibody-wb-pc12-cells.JPG)
Western blot - Anti-Mre11 antibody [12D7] (ab214)Anti-Mre11 antibody [12D7] (ab214) at 1/500 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Secondary
Anti-mouse IgG HRP-conjugated antibody
Developed using the ECL technique.
Predicted band size: 79 kDa7.5% SDS-PAGE
The signal was developed with Trident ECL plus-Enhanced.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Mre11 antibody [12D7] (ab214)](https://www.abcam.co.jp/ps/products/0/ab214/Images/ab214-275735-anti-mre11-antibody-12d7-western-blot.png)
Western blot - Anti-Mre11 antibody [12D7] (ab214)All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Human Mre-11-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : anti-mouse IgG HRP-conjugated antibody
Predicted band size: 79 kDaThis image was generated using the ascites version of the product.
7.5% SDS-PAGE
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![Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)](https://www.abcam.co.jp/ps/products/0/ab214/Images/abcam214_1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)Image supplied by Dr Domenico Delia, Istituto Nazionale Tumori, Italy.Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells.
(Panel D - negative control).This image was generated using the ascites version of the product.
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![Western blot - Anti-Mre11 antibody [12D7] (ab214)](https://www.abcam.co.jp/ps/products/0/ab214/Images/ab214-208986-anti-mre11-antibody-12d7-western-blot.jpg)
Western blot - Anti-Mre11 antibody [12D7] (ab214)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/500 dilution
Lane 1 : Mouse breast cancer cell line - whole cell lysate. Transfected with vector control.
Lane 2 : Mouse breast cancer cell line - whole cell lysate. Transfected with knockdown shRNA targeting Mre11 gene.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-mouse IgG polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 79 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis image was generated using the ascites version of the product.
プロトコール
参考文献 (71)
ab214 は 71 報の論文で使用されています。
- Mooser C et al. Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nat Commun 11:123 (2020). PubMed: 31913317
- Onn L et al. SIRT6 is a DNA double-strand break sensor. Elife 9:N/A (2020). PubMed: 31995034
- Thuault S et al. A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells. Sci Rep 10:6787 (2020). PubMed: 32321993
- Wang J et al. Elevated MRE11 expression associated with progression and poor outcome in prostate cancer. J Cancer 10:4333-4340 (2019). PubMed: 31413753
- Nieminuszczy J et al. EXD2 Protects Stressed Replication Forks and Is Required for Cell Viability in the Absence of BRCA1/2. Mol Cell 75:605-619.e6 (2019). PubMed: 31255466
