Name: Anti-Mre11 antibody [12D7]
SKU: ab214
Rating: 5 (10 reviews)

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一次抗体 - Mouse Anti-Mre11 antibody [12D7] (ab214)
Flow Cyt, ICC/IF, IHC-Fr, WB, IP, IHC-P

二次抗体 - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
WB, IP, ELISA, IHC-P

タンパク質 - Recombinant Human Mre11 protein (ab114277)
ELISA, SDS-PAGE, WB

関連製品

データシート
文献 (57)
プロトコール

製品名

Anti-Mre11 antibody [12D7]
Mre11 一次抗体製品一覧
製品の詳細

Mouse monoclonal [12D7] to Mre11
由来種

Mouse
アプリケーション

適用あり: Flow Cyt, ICC/IF, IHC-Fr, WB, IP, IHC-Pmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Amino acids 182-582 of Mre11 expressed in E. coli.
ポジティブ･コントロール
- T24 cells, recombinant fusion protein.
特記事項

This product was changed from ascites to tissue culture supernatant on 10^th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファー

Constituent: PBS
Concentration information loading...
精製度

Tissue culture supernatant
ポリ/モノ

モノクローナル
クローン名

12D7
ミエローマ

NS1
アイソタイプ

IgG1
軽鎖の種類

kappa
研究分野

Compatible Secondaries
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
- Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
Recombinant Protein
- Recombinant Human Mre11 protein (ab114277)

Our Abpromise guarantee covers the use of ab214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション	Abreviews	特記事項
Flow Cyt		Use 1-2µg for 10⁶ cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF		1/200 - 1/500. Wash cells with PBS, then wash with PBS containing 0.5% Triton X-100 and fix for 5 min with PBS containing 3% paraformaldehyde. Block cells for 1 h in PBS containing 15% fetal bovine serum (see Robinson et al).
IHC-Fr		1/200.
WB		Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). (see Robinson et al).
IP		Use a concentration of 1 - 5 µg/ml. (for normal lymphoblastoid cell lines).
IHC-P		Use at an assay dependent concentration.

機能

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
関連疾患

Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
配列類似性

Belongs to the MRE11/RAD32 family.
翻訳後修飾

Phosphorylated upon DNA damage, probably by ATM or ATR.
細胞内局在

Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
Target information above from: UniProt accession P49959 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 4361 Human
- Entrez Gene: 17535 Mouse
- Entrez Gene: 64046 Rat
- Omim: 600814 Human
- SwissProt: P49959 Human
- SwissProt: Q61216 Mouse
- SwissProt: Q9JIM0 Rat
- Unigene: 192649 Human
- Unigene: 149071 Mouse
- Unigene: 209040 Rat
see all
別名
- AT like disease antibody
- Ataxia telangiectasia disorder like antibody
- ATLD antibody
- DNA recombination and repair protein antibody
- Double strand break repair protein MRE11A antibody
- Double-strand break repair protein MRE11A antibody
- endo/exonuclease Mre11 antibody
- HNGS1 antibody
- meiotic recombination (S. cerevisiae) 11 homolog A antibody
- Meiotic recombination 11 homolog 1 antibody
- meiotic recombination 11 homolog A (S. cerevisiae) antibody
- Meiotic recombination 11 homolog A antibody
- MmMRE11A antibody
- Mre 11 antibody
- MRE 11a antibody
- MRE 11b antibody
- MRE11 homolog 1 antibody
- MRE11 homolog A antibody
- MRE11 homolog double strand break repair nuclease antibody
- MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
- MRE11 meiotic recombination 11 homolog A antibody
- MRE11_HUMAN antibody
- MRE11A antibody
- MRE11b antibody
- OTTHUMP00000236830 antibody
- OTTHUMP00000236831 antibody
- OTTHUMP00000236832 antibody
- OTTHUMP00000236833 antibody
see all

Western blot - Anti-Mre11 antibody [12D7] (ab214)

Western blot of PC12 (brain)whole cell extracts at 30µg per lane, labeling Mre11 with ab214 at 1:500. A HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

This image was generated using the ascites version of the product.
Western blot - Anti-Mre11 antibody [12D7] (ab214)

All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/1000 dilution

Lane 1 : 293T whole cell extract
Lane 2 : Transfected 293T whole cell extract

Lysates/proteins at 30 µg per lane.

Predicted band size: 79 kDa

This image was generated using the ascites version of the product.
Immunohistochemistry (Frozen sections) - Anti-Mre11 antibody [12D7] (ab214)This image is courtesy of an anonymous Abreview

ab214 staining Mre11 in human normal and gastric cancer tissue sections (20µm) by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde for 3 hours, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/200 in 3% BSA + 0.1% Triton X-100 in TBS buffer) for 12 hours at 4°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

This image was generated using the ascites version of the product.
See Abreview
Western blot - Anti-Mre11 antibody [12D7] (ab214)This image is courtesy of an anonymous Abreview

All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/500 dilution

Lane 1 : Mouse breast cancer cell line - whole cell lysate. Transfected with vector control.
Lane 2 : Mouse breast cancer cell line - whole cell lysate. Transfected with knockdown shRNA targeting Mre11 gene.

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP-conjugated Goat anti-mouse IgG polyclonal at 1/1000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 79 kDa
Observed band size: 75 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

This image was generated using the ascites version of the product.
See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)Image supplied by Dr Domenico Delia, Istituto Nazionale Tumori, Italy.

Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells.
(Panel D - negative control).

This image was generated using the ascites version of the product.
Western blot - Anti-Mre11 antibody [12D7] (ab214)

This picture was kindly supplied as part of the reviews for ab214 and for ab89 submitted by Anya Polischouk.

The bands represent nuclear (N) and cytoplasmic (C) extract from human lung cancer cells (U1810).

This image was generated using the ascites version of the product.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [12D7] (ab214)This image is courtesy of an anonymous Abreview

ab214 at 1/200 staining normal human bronchus tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in EDTA was peformed. The tissue was blocked in normal rabbit serum and then incubated with the antibody for 1 hour. An HRP conjugated goat polyclonal antibody was used as the secondary.

This image was generated using the ascites version of the product.
See Abreview
Flow Cytometry - Anti-Mre11 antibody [12D7] (ab214)

Overlay histogram showing HeLa cells stained with ab214 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab214, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

This image was generated using the ascites version of the product.

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

This product has been referenced in:

Walker AK et al. MRE11 as a Predictive Biomarker of Outcome After Radiation Therapy in Bladder Cancer. Int J Radiat Oncol Biol Phys N/A:N/A (2019). Read more (PubMed: 30885775) »
Cowman S et al. Decrease of Nibrin expression in chronic hypoxia is associated with hypoxia-induced chemoresistance in some brain tumour cells. BMC Cancer 19:300 (2019). Read more (PubMed: 30943920) »

See all 57 Publications for this product

ab214 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-10 of 11 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Pig Cell (LLC-PK1 cell line)

Permeabilization

Yes - 0.5% Triton-X 100

Specification

LLC-PK1 cell line

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: R.T°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 Feb 10 2016

Application

Immunohistochemistry (Frozen sections)

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Sample

Human Tissue sections (Normal or gastric cancer tissues)

Specification

Normal or gastric cancer tissues

Permeabilization

Yes - 0.1% Triton X-100

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 Feb 20 2014

Application

Immunocytochemistry

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C

Sample

Mouse Cultured Cells (Mouse embryonic fibroblasts)

Specification

Mouse embryonic fibroblasts

Permeabilization

Yes - 0.1% Triton X-100

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 Feb 19 2014

Application

Immunocytochemistry

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

Sample

Human Cultured Cells (Hela cell line)

Specification

Hela cell line

Permeabilization

Yes - 0.1% Triton X-100

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 Feb 18 2014

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C

Sample

Human Cell (Human colon cancer cell lines)

Specification

Human colon cancer cell lines

Permeabilization

Yes - 0.3% Triton X-100

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 Oct 15 2013

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (7.5)

Sample

Mouse Cell lysate - whole cell (Mouse breast cancer cell line)

Specification

Mouse breast cancer cell line

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 Oct 15 2013

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)

Loading amount

15 µg

Specification

Mouse embryonic fibroblasts

Gel Running Conditions

Reduced Denaturing (10% Tris-Gly gel)

Blocking step

LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

Abcam user community

Verified customer

投稿 Nov 08 2011

Application

Western blot

Sample

Human Cell lysate - nuclear (human cancer cells (colon, bladder))

Loading amount

20 µg

Specification

human cancer cells (colon, bladder)

Gel Running Conditions

Reduced Denaturing (4-12)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C

Abcam user community

Verified customer

投稿 Apr 12 2011

Application

Western blot

Sample

Human Cell lysate - whole cell (HeLa cells)

Loading amount

20 µg

Specification

HeLa cells

Gel Running Conditions

Reduced Denaturing (10% Tris-Gly gel)

Blocking step

LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

Abcam user community

Verified customer

投稿 Nov 17 2010

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Normal Bronchus)

Specification

Normal Bronchus

Fixative

Formaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: EDTA

Permeabilization

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 5%

Abcam user community

Verified customer

投稿 Jun 15 2007

1-10 of 11 Abreviews or Q&A

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

ミエローマ

アイソタイプ

軽鎖の種類

研究分野

関連製品

Compatible Secondaries

Isotype control

Recombinant Protein

アプリケーション

ターゲット情報

機能

関連疾患

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

参考文献

レビューと Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Mre11 antibody [12D7]

Immunohistochemistry (Frozen sections) abreview for Anti-Mre11 [12D7] antibody

Immunocytochemistry abreview for Anti-Mre11 [12D7] antibody

Immunocytochemistry abreview for Anti-Mre11 [12D7] antibody

Immunocytochemistry/ Immunofluorescence abreview for Anti-Mre11 [12D7] antibody

Western blot abreview for Anti-Mre11 antibody [12D7]

Western blot abreview for Anti-Mre11 antibody [12D7]

Western blot abreview for Anti-Mre11 antibody [12D7]

Western blot abreview for Anti-Mre11 antibody [12D7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mre11 antibody [12D7]