Mouse Von Willebrand Factor A2 Matched 抗体 Pair Kit (ab213466)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 62.5 pg/ml - 4000 pg/ml
製品の概要
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製品名
Mouse Von Willebrand Factor A2 Matched Antibody Pair Kit
Von Willebrand Factor キット 製品一覧 -
検出方法
Colorimetric -
アッセイタイプ
ELISA set -
検出感度
5.7 pg/ml -
検出範囲
62.5 pg/ml - 4000 pg/ml -
種交差性
交差種: Mouse -
製品の概要
Mouse Von Willebrand Factor 2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Von Willebrand Factor 2.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab208980. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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アプリケーション
適用あり: ELISA, IAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 5 x 96 tests Mouse Von Willebrand Factor 2 Capture Antibody 1 x 50µg Mouse Von Willebrand Factor 2 Detector Antibody 1 x 12.5µg Mouse Von Willebrand Factor 2 Lyophilized Protein 1 vial -
研究分野
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機能
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. -
組織特異性
Plasma. -
関連疾患
Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. -
配列類似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
ドメイン
The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. -
翻訳後修飾
All cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated. -
細胞内局在
Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules. - Information by UniProt
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別名
- Coagulation factor VIII
- Coagulation factor VIII VWF
- F8VWF
see all -
参照データベース
- Entrez Gene: 22371 Mouse
- SwissProt: Q8CIZ8 Mouse
- Unigene: 22339 Mouse
関連製品
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Alternative Versions
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Matched antibody pairs
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab213466の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IA |
Use at an assay dependent concentration.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
IA
Use at an assay dependent concentration. |
画像
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Standard calibration curve. Background subtracted values are graphed.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab213466 は論文での使用が確認できていません。