Mouse VCAM1 ELISA Kit (ab100750)
Key features and details
- Sensitivity: 1.5 pg/ml
- Range: 2.05 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse VCAM1 ELISA Kit
VCAM1 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 1.5 pg/ml -
検出範囲
2.05 pg/ml - 500 pg/ml -
添加回収試験
96 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 97.36 84% - 107% Serum 96.43 82% - 105% Plasma 94.64 81% - 104% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s VCAM1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse VCAM1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse VCAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and VCAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse VCAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VCAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 120X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 2 x 30ml Biotinylated anti-Mouse VCAM1 2 vials Recombinant Mouse VCAM1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml VCAM1 Microplate (12 strips x 8 wells) 1 unit -
研究分野
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機能
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. -
組織特異性
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. -
配列類似性
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. -
ドメイン
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. -
翻訳後修飾
Sialoglycoprotein. -
細胞内局在
Membrane. - Information by UniProt
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別名
- CD106
- CD106 Antigen
- INCAM 100
see all -
参照データベース
- Entrez Gene: 22329 Mouse
- SwissProt: P29533 Mouse
- Unigene: 440909 Mouse
- Unigene: 76649 Mouse
画像
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Representative Standard Curve
Representative Standard Curve using ab100750
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Representative Standard CurveRepresentative Standard Curve using ab100750
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab100750 は 5 報の論文で使用されています。
- Hamzaoui M et al. Soluble Epoxide Hydrolase Inhibition Prevents Experimental Type 4 Cardiorenal Syndrome. Front Mol Biosci 7:604042 (2020). PubMed: 33777999
- Button EB et al. ApoA-I deficiency increases cortical amyloid deposition, cerebral amyloid angiopathy, cortical and hippocampal astrogliosis, and amyloid-associated astrocyte reactivity in APP/PS1 mice. Alzheimers Res Ther 11:44 (2019). PubMed: 31084613
- Wang Y et al. Role of the Rho/ROCK signaling pathway in the protective effects of fasudil against acute lung injury in septic rats. Mol Med Rep 18:4486-4498 (2018). PubMed: 30221694
- Yao L et al. Obesity-induced vascular inflammation involves elevated arginase activity. Am J Physiol Regul Integr Comp Physiol 313:R560-R571 (2017). PubMed: 28835451
- Cavalera M et al. Dietary rose hip exerts antiatherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice. J Nutr Biochem 44:52-59 (2017). Functional Studies ; Mouse . PubMed: 28399420