Mouse uPA Total ELISA Kit (URK) (ab198512)
製品の概要
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製品名
Mouse uPA Total ELISA Kit (URK)
uPA キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Known Conc 20 0.06ng/ml 0.005 8.12% Known Conc 20 0.3ng/ml 0.019 6.18% Known Conc 20 3.01ng/ml 0.112 3.73% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Known Conc 10 0.09ng/ml 0.008 8.52% Known Conc 10 0.25ng/ml 0.019 7.47% Known Conc 10 2.66ng/ml 0.154 5.79% -
サンプルの種類
Plasma -
アッセイタイプ
Quantitative -
検出感度
0.01 ng/ml -
検出範囲
0.01 ng/ml - 10 ng/ml -
添加回収試験
97.75 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Spike = 97.7 89.3% - 106% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse
非交差種: Sheep -
製品の概要
Abcam’s uPA Total (URK) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of total urokinase plasminogen activator antigen in mouse plasma.
Mouse uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-murine uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then bound by the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-mouse uPA primary antibody 1 vial Anti-rabbit horseradish peroxidase secondary antibody 1 vial Mouse uPA standard 1 vial TMB Substrate Solution 1 x 10ml uPA Antibody pre-coated 96 well microplate (12 x 8 well strips) 1 x 96 tests -
研究分野
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関連性
Urokinase type plasminogen activator (uPA) is secreted from cells as a precursor which is activated to the two chain form consisting of an A and B chain. The active high MW form is further processed by removal of an amino terminal fragment to an active low MW form (35 kDa). uPA is a serine protease that activates plasminogen toplasmin. High levels of uPA and plasminogen activator inhibitor type 1 (PAI 1) in breast cancer tissue extracts have been associated with rapid disease progression. The malignant phenotype of prostatic tumor cells correlates with the expression of both uPA and its cell membrane receptor (uPAR). -
別名
- ATF
- BDPLT5
- EC 3.4.21.73
see all -
参照データベース
- Entrez Gene: 18792 Mouse
- SwissProt: P06869 Mouse
- Unigene: 4183 Mouse
アプリケーション
Our Abpromise guarantee covers the use of ab198512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
画像
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Standard Curve.Full range standard curve using ab198512 uPA Total (URK) Mouse ELISA Kit.
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Standard Curve.Linear range standard curve using ab198512 uPA Total (URK) Mouse ELISA Kit.
プロトコール
参考文献
ab198512 has not yet been referenced specifically in any publications.
