Mouse Thrombopoietin ELISA Kit (ab100748)
Key features and details
- Sensitivity: 30 pg/ml
- Range: 27.4 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse Thrombopoietin ELISA Kit
Thrombopoietin キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 30 pg/ml -
検出範囲
27.4 pg/ml - 20000 pg/ml -
添加回収試験
94 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 96.23 83% - 103% Serum 93.45 82% - 103% Plasma 94.28 84% - 104% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s Thrombopoietin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Thrombopoietin in serum, plasma and cell culture supernates.
This assay employs an antibody specific for Mouse Thrombopoietin coated on a 96-well plate. Standards and samples are pipetted into the wells and Thrombopoietin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Thrombopoietin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thrombopoietin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse Thrombopoietin 2 vials Recombinant Mouse Thrombopoietin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml Thrombopoietin Microplate (12 x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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関連性
Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported. -
細胞内局在
Secreted -
別名
- C mpl ligand
- Megakaryocyte colony stimulating factor
- Megakaryocyte growth and development factor
see all -
参照データベース
- SwissProt: P40226 Mouse
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab100748 は 2 報の論文で使用されています。
- Zhang Y et al. Chemically modified in-vitro-transcribed mRNA encoding thrombopoietin stimulates thrombopoiesis in mice. Mol Ther Nucleic Acids 29:657-671 (2022). PubMed: 36090760
- Yan R et al. Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression. Cell Death Dis 12:955 (2021). PubMed: 34657146