製品の概要

  • 製品名

    Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT)
    Thrombin-Antithrombin Complex キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 9.8%
  • サンプルの種類

    Cell culture supernatant, Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    > 0.01 ng/ml
  • 検出範囲

    0.031 ng/ml - 8 ng/ml
  • 添加回収試験

    97 %

  • 全工程の試験時間

    5h 00m
  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Mouse
  • 製品の概要

    TAT Complexes Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TAT Complexes levels in plasma and cell culture supernatants.


    A TAT Complexes specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TAT Complexes specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TAT Complexes captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Mouse TAT Complexes Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
    TAT Complexes Microplate (12 x 8 well strips) 1 unit
    TAT Complexes Standard 1 x 2 vials
  • 研究分野

  • 関連性

    Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Antithrombin: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. Antithrombin inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • 細胞内局在

    Secreted
  • 別名

    • antithrombin III
    • AT3
    • AT3D
    • ATIII
    • coagulation factor II (thrombin)
    • F2
    • PRO0309
    • prothrombin
    • prothrombin B chain
    • PT
    • RPRGL2
    • serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
    • serine protease
    • serine-cysteine proteinase inhibitor clade C member 1
    • serpin C1
    • serpin peptidase inhibitor, clade C (antithrombin), member 1
    • SERPINC1
    • TAT
    • THPH1
    • THPH7
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab137994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Mouse TAT measured in murine biological fluids (diluted 1/256; duplicates +/- SD).

  • Representative Standard Curve using ab137994

参考文献

This product has been referenced in:

  • Ainsworth S  et al. The paraspecific neutralisation of snake venom induced coagulopathy by antivenoms. Commun Biol 1:34 (2018). Read more (PubMed: 30271920) »
  • Hayashi H  et al. Sirt3 deficiency does not affect venous thrombosis or NETosis despite mild elevation of intracellular ROS in platelets and neutrophils in mice. PLoS One 12:e0188341 (2017). Read more (PubMed: 29236713) »
See all 3 Publications for this product

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