製品の概要

  • 製品名

    Mouse TGF beta 1 ELISA Kit
    TGF beta 1 キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4 7.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 4 5.8%
  • サンプルの種類

    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    7.8 pg/ml
  • 検出範囲

    31.3 pg/ml - 2000 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 124% - 141%
    Serum 92% - 103%

  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Mouse
  • 製品の概要

    Abcam’s TGF beta 1 Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Mouse TGF beta 1 concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate).

    Mouse TGF beta 1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Mouse TGF beta 1 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mouse TGF beta 1 captured on the plate

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Assay Buffer Concentrate 2 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 6 units
    Biotinylated mouse anti-TGF beta 1 monoclonal antibody 1 x 120µl
    Microplate coated with monoclonal antibody to mouse TGF beta 1 (12 x 8 wells) 1 unit
    Mouse TGF beta 1 Standard lyophilized 2 vials
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • 研究分野

  • 機能

    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • 組織特異性

    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • 関連疾患

    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • 配列類似性

    Belongs to the TGF-beta family.
  • 翻訳後修飾

    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • 細胞内局在

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 別名

    • Camurati-Engelmann disease
    • CED
    • DPD1
    • LAP
    • Latency-associated peptide
    • TGF beta 1
    • TGF beta 1 protein
    • TGF-beta 1 protein
    • TGF-beta-1
    • TGFB
    • tgfb1
    • TGFB1_HUMAN
    • TGFbeta
    • Transforming growth factor beta 1
    • transforming growth factor beta-1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Standard curve of Ms TGFb, with background signal subtracted (duplicates; +/- SD).
  • Mouse TGFb measured in activated biological fluids, background signal subtracted (duplicates +/- SD). * Samples without activation.
  • Representative Standard Curve using ab119557.

プロトコール

参考文献

This product has been referenced in:

  • Yang X  et al. Precision toxicology shows that troxerutin alleviates ochratoxin A-induced renal lipotoxicity. FASEB J 33:2212-2227 (2019). Read more (PubMed: 30247986) »
  • You P  et al. Local level of TGF-ß1 determines the effectiveness of dexamethasone through regulating the balance of Treg/Th17 cells in TNBS-induced mouse colitis. Exp Ther Med 15:3639-3649 (2018). Read more (PubMed: 29545894) »
See all 18 Publications for this product

レビューと Q&A

1-6 of 6 Abreviews or Q&A

Answer


ab108792: We have never tested brain tissue supernatants so I do not know if it would work. I cannot recommend the dilution range.

ab119557: Again, we have not tested brain tissue with this kit but there is a publication where a FlowCytomix kit, which uses the same antibodies, was used for brain tissues. Therefore, I would assume that this kit would work on your samples however, I cannot guarantee this.

The publication is the following and may be of interest to you:

Nogo-Receptors NgR1 and NgR2 Do Not Mediate Regulation of CD4 T Helper Responses and CNS Repair in Experimental Autoimmune Encephalomyelitis, Karin Steinbach et al, http://www.plosone.org 8 November 2011 | Volume 6 | Issue 11 | e26341

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ELISA performed on Kidney tissue samples

Excellent Good 4/5 (Ease of Use)
Abreviews
The kit is a bit of a long assay, but the results were great. There was a definite upregulation of TGFb in the inflamed kidney tissue.

Dr. Liad Hinden

Verified customer

投稿 Sep 16 2019

ELISA used on kidney tissue samples

Excellent Good 4/5 (Ease of Use)
Abreviews
The assay was used on wt mice vs db/db mice, which are obese and diabetic. The mice also suffer from chronic kidney damage. The graph shows the expected result, there is an upregulation in TGFb in db/db mice kidney tissue.

Anna Permyakova

Verified customer

投稿 Sep 13 2019

Cross-reactivity with human TGF-b1

Inconclusive Average 3/5 (Ease of Use)
Abreviews
As we plan to do TGF-b1 determinations from supernatants of human and mice co-cultured cells, we checked the ability of this Kit to distinguish both forms, human and rodent.

We measured samples of DMEM, DMEM 10% and 0,5% FBS and DMEM + purified hTGF-b1 protein. Although samples with low FBS gives us a low value of TGF, samples with hTGF-b1 gives very strong signal.

We consider that specificity of the kit is not promised, so we want a discount check for the value of the Kit. Also we want to notice that we buy two different Kit to be absolute sure that the Kit is the problem not our manipulation, so we claim for both Kits...



Despite of the Kit is very easy to use, we consider that detection is a long process due to a lot steps of washing comparing to hTGFb1 detection Kit.

Abcam user community

Verified customer

投稿 Oct 28 2013

Answer






We do have reports concerning tissue samples for ab119557 indicating that lysis buffers containing 0.5% TritonX-100 (detergent) had no deleterious effect, but we did not test this ourselves.


We also have not tested the effect of protease and phosphatase inhibitors.

We recommend, that - as a positive control- you spike in some of the standard at a known concentration in your buffer and see if it performs as expected.

The lysis buffer only should be used a negative control.

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Answer

Thank you for contacting us.

The TGF beta 1 Mouse ELISA Kit (with color giving dyes) (ab119557) is the only kit we currently have which can be used to detect TGF beta 1 in mouse samples. Regrettably we have not as yet tested this kit with tissue samples. It may be that the kit would be compatible with such samples but we would not be able toguarentee this.

I am very sorry that I could not be of more help. If you have any further questions, please do not hesitate to contact us again.

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