製品の概要

  • 製品名

    Mouse TFPI ELISA Kit
    TFPI キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Mouse serum 8 3.7%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Mouse serum 3 7.2%
  • サンプルの種類

    Cell culture supernatant, Urine, Serum, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    1.7 pg/ml
  • 検出範囲

    18.75 pg/ml - 1200 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Urine 99 97% - 102%
    Serum 102 96% - 109%
    Tissue Extracts 92 85% - 101%
    Cell culture media 98 96% - 101%
    Heparin Plasma 109 104% - 112%
    EDTA Plasma 101 96% - 105%
    Citrate Plasma 103 98% - 111%

  • 全工程の試験時間

    1h 30m
  • ステップ

    One step assay
  • 種交差性

    交差種: Mouse
    非交差種: Rat, Cow, Human
  • 製品の概要

    TFPI in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse TFPI protein in serum, plasma, urine, cell culture supernatant, and tissue extract samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples in 1X Cell Extraction Buffer PTR - 1.7 pg/mL


    Samples in Sample Diluent NS - 1.87 pg/mL


     

  • 特記事項

    Mouse TFPI (tissue factor pathway inhibitor) is a secreted protein located on the endothelial surface, within platelets, in plasma, and on monocytes. Functionally, mouse TFPI is the primary inhibitor of intravascular tissue factor (TF) activity. TF is the principal protein that initiates blood coagulation in vivo. Thus, Mouse TFPI inhibits coagulation.

    Mouse TFPI has an acidic N-terminal region, three Kunitz (K) domains separated by two linker regions, and a C-terminal basic region. The first K domain inhibits TF from binding coagulation factor VIIa. The second K domain inhibits factor Xa. The third K domain binds to heparin. Human and rat TFPI exhibit 58% and 71% amino acid identity to mouse TFPI, respectively.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    Mouse TFPI Lyophilized Recombinant Protein 2 vials
    10X Mouse TFPI Detector Antibody 1 x 600µl
    10X Mouse TFPI Capture Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPR 1 x 6ml
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • 研究分野

  • 機能

    Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
  • 組織特異性

    Mostly in endothelial cells.
  • 配列類似性

    Contains 3 BPTI/Kunitz inhibitor domains.
  • ドメイン

    This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
  • 翻訳後修飾

    O-glycosylated.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • Anti convertin
    • EPI
    • Extrinsic pathway inhibitor
    • LACI
    • Lipoprotein associated coagulation inhibitor
    • Lipoprotein-associated coagulation inhibitor
    • TFI
    • TFPI
    • TFPI 1
    • TFPI1
    • TFPI1_HUMAN
    • Tissue factor pathway inhibitor
    • Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor)
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab217776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of TFPI were measured in duplicates, interpolated from the TFPI standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:100, plasma (citrate) 1:400, plasma (EDTA) 1:400 and plasma (heparin) 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFPI concentration was determined to be 57.10 ng/mL in neat serum, 241.24 ng/mL in neat plasma (citrate), 259.59 ng/mL in neat plasma (EDTA) and 303.08 ng/mL in neat plasma (heparin).

  • The concentrations of TFPI were measured in duplicates, interpolated from the TFPI standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50%, and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFPI concentration was determined to be 120.64 pg/mL in native, neat urine, 1,353.96 pg/mL in spiked, neat urine and 1,130.58 pg/mL in spiked, neat cell culture media.

  • The concentrations of TFPI were measured in duplicate and interpolated from the TFPI standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFPI concentration was determined to be 525.44 pg/mL in mouse heart extract and 69.55 pg/mL in mouse liver extract.

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参考文献

ab217776 has not yet been referenced specifically in any publications.

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