Mouse Resistin ELISA Kit (ab205574)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.5 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse Resistin ELISA Kit
Resistin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 8 5.1% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 3 7.5% -
サンプルの種類
Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts -
アッセイタイプ
Sandwich (quantitative) -
検出感度
6.5 pg/ml -
検出範囲
31.25 pg/ml - 2000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 91 88% - 95% Cell culture media 100 98% - 103% Hep Plasma 104 100% - 111% EDTA Plasma 95 90% - 103% Cit plasma 109 106% - 115% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse
非交差種: Pig -
製品の概要
Mouse Resistin ELISA Kit (ab205574) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Resistin protein in cell culture extracts, cell culture supernatant, plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Resistin with 6.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Mouse Resistin (RETN), also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone), is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family. Mouse Resistin is a 114-amino acid (aa) peptide (with a 20 aa signal sequence and a 94 aa mature segment) containing 11 cysteines that allow the association of several Resistin monomers into macromolecular complexes. Mouse Resistin is 75% and 56% identical to rat and human Resistin, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Mouse Resistin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Mouse Resistin Capture Antibody (Lyophilized) 1 vial Mouse Resistin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. -
組織特異性
Expressed only in fatty tissues. -
配列類似性
Belongs to the resistin/FIZZ family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Adipose tissue specific secretory factor
- Adipose tissue-specific secretory factor
- ADSF
see all -
参照データベース
- Entrez Gene: 57264 Mouse
- SwissProt: Q99P87 Mouse
- Unigene: 1181 Mouse
関連製品
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Alternative Versions
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Standard curve comparison between mouse Resistin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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Native mouse Resistin was measured in 2.5% mouse serum diluted in a 2-fold dilution series in Sample Diluent NS. Recombinant mouse Resistin was spiked into 10% cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Resistin were measured in duplicate and interpolated from the mouse Resistin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
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Native mouse Resistin was measured in 2.5% mouse plasma citrate, mouse plasma EDTA, and mouse plasma heparin samples diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Resistin were measured in duplicate and interpolated from the mouse Resistin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
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Native mouse Resistin was measured in 10 μg/mL of 3T3-L1 adipocyte cell extract diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. The concentrations of mouse Resistin were measured in duplicate and interpolated from the mouse Resistin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
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3T3-L1 cells were cultured in the presence and absence 1 µM Dexamethasone, 0.5 mM IBMX, 1 µg/mL insulin for 10 days to generate adipocytes and undifferentiated cells respectively. At the end of the treatment, protein extraction was carried out according to section 11.4 of the booklet. Both 3T3-L1 adipocytes and undifferentiated cell extracts were measured in duplicate at 10 µg/mL using this kit. The Raw O.D. values for each sample are graphed, with the background O.D. shown as the dashed line. The 3T3-L1 adipocytes were measured at 179 pg of Resistin/µg of cell lysate, whereas the 3T3-L1 undifferentiated cells measured below the limit of detection for this assay.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab205574 は 2 報の論文で使用されています。
- Pellegrini C et al. NLRP3 at the crossroads between immune/inflammatory responses and enteric neuroplastic remodelling in a mouse model of diet-induced obesity. Br J Pharmacol 178:3924-3942 (2021). PubMed: 34000757
- Cheng CF et al. Adipocyte browning and resistance to obesity in mice is induced by expression of ATF3. Commun Biol 2:389 (2019). PubMed: 31667363