製品の概要

  • 製品名

    Mouse RANTES ELISA Kit
    RANTES キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 8 6.5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 3 4.2%
  • サンプルの種類

    Cell culture supernatant, Serum
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    5.04 pg/ml
  • 検出範囲

    7.81 pg/ml - 500 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 103 99% - 105%
    Serum 117 111% - 123%

  • 全工程の試験時間

    1h 30m
  • ステップ

    One step assay
  • 種交差性

    交差種: Mouse
    非交差種: Cow
  • 製品の概要

    RANTES in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of RANTES protein in mouse serum, platelet poor plasma, and cell culture supernatant.


    The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • 特記事項

    RANTES is a small chemoattractant for monocytes, eosinophils, basophils, and some T cells. RANTES (also known as CCL5) is produced by a variety of cells including endothelium, fibroblasts, respiratory epithelium, smooth muscle, astrocytes, platelets, eosinophils, keratinocytes, and CD4+ and CD8+ T cells in response to cytokine stimulation.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    20X Mouse RANTES Capture Antibody 1 x 330µl
    20X Mouse RANTES Detector Antibody 1 x 330µl
    Antibody Diluent 5BI 1 x 6ml
    Mouse RANTES Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • 研究分野

  • 機能

    Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
  • 組織特異性

    T-cell and macrophage specific.
  • 配列類似性

    Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾

    N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
    The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • Beta chemokine RANTES
    • Beta chemokine RANTES precursor
    • C C motif chemokine 5
    • CCL 5
    • CCL5
    • CCL5_HUMAN
    • Chemokine (C C motif) ligand 5
    • Chemokine CC Motif Ligand 5
    • D17S136E
    • EoCP
    • Eosinophil chemotactic cytokine
    • MGC17164
    • RANTES(4-68)
    • Regulated upon activation normally T expressed and presumably secreted
    • SCYA 5
    • SCYA5
    • SIS delta
    • SIS-delta
    • SISd
    • Small inducible cytokine A5
    • Small inducible cytokine A5 (RANTES)
    • Small inducible cytokine subfamily A (Cys Cys) member 5
    • Small-inducible cytokine A5
    • T cell specific protein p288
    • T cell specific protein RANTES
    • T cell specific RANTES protein
    • T cell-specific protein P228
    • T-cell-specific protein RANTES
    • TCP 228
    • TCP228
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab215537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

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  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between mouse RANTES SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of RANTES were measured in duplicates, interpolated from the RANTES standard curves and corrected for sample dilution. Undiluted samples are as follows: RAW 264.7 + LPS supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANTES concentration was determined to be 5,081 pg/mL in RAW 264.7 + LPS supernatant.

  • The concentrations of RANTES in rat serum were measured in duplicate, interpolated from the mouse RANTES standard curves and corrected for sample dilution. Undiluted samples are rat serum extract 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean RANTES concentration was determined to be 3,492 pg/mL in rat serum.

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参考文献

This product has been referenced in:

See 1 Publication for this product

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