Mouse Prolactin ELISA Kit (ab100736)
Key features and details
- Sensitivity: 30 pg/ml
- Range: 27.43 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse Prolactin ELISA Kit
Prolactin キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 30 pg/ml -
検出範囲
27.43 pg/ml - 20000 pg/ml -
添加回収試験
81 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 76.85 68% - 87% Serum 82.47 73% - 94% Plasma 76.78 67% - 85% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s Prolactin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Prolactin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse Prolactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Prolactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Prolactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Prolactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Assay Diluent C 1 x 30ml Biotinylated anti-Mouse Prolactin 2 vials Prolactin Microplate (12 x 8 wells) 1 unit Recombinant Mouse Prolactin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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関連性
Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin acts primarily on the mammary gland by promoting lactation. -
細胞内局在
Secreted -
別名
- AV290867
- Lactogenic Hormone
- Luteotropic Hormone
see all -
参照データベース
- Entrez Gene: 19109 Mouse
- SwissProt: P06879 Mouse
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab100736 は 16 報の論文で使用されています。
- Mellado S et al. Prolactin Reduces Hippocampal Parvalbumin and GABAA Receptor Expression in Female Mice. Neuroendocrinology 112:796-806 (2022). PubMed: 34666336
- Masuda S et al. Maternal prolactin levels during late pregnancy and nurturing behavior of offspring in mice. Dev Psychobiol 64:e22264 (2022). PubMed: 35312052
- Gotlieb N et al. Impact of Chronic Prenatal Stress on Maternal Neuroendocrine Function and Embryo and Placenta Development During Early-to-Mid-Pregnancy in Mice. Front Physiol 13:886298 (2022). PubMed: 35770190
- Grabrucker S et al. Activation of the medial preoptic area (MPOA) ameliorates loss of maternal behavior in a Shank2 mouse model for autism. EMBO J 40:e104267 (2021). PubMed: 33491217
- Li D et al. Oxytocin receptor induces mammary tumorigenesis through prolactin/p-STAT5 pathway. Cell Death Dis 12:588 (2021). PubMed: 34099636