Mouse PF4 ELISA Kit (CXCL4) (ab100735)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 4.12 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse PF4 ELISA Kit (CXCL4)
PF4 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 6 pg/ml -
検出範囲
4.12 pg/ml - 3000 pg/ml -
添加回収試験
100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 73.99 67% - 83% Serum 118.2 104% - 127% Plasma 109.3 92% - 129% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s PF4 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse PF4 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse PF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and PF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse PF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-mouse PF4 2 vials PF4 Microplate (12 x 8 wells) 1 unit Recombinant Mouse PF4 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
Binds non-covalently to a proteoglycan molecule. -
細胞内局在
Secreted. - Information by UniProt
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別名
- C-X-C motif chemokine 4
- Chemokine (C X C motif) ligand 4
- Chemokine (CXC motif) ligand 4
see all -
参照データベース
- Entrez Gene: 56744 Mouse
- SwissProt: Q9Z126 Mouse
画像
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Sandwich ELISA - PF4 (CXCL4) Mouse ELISA Kit (ab100735)
PF4 measured in cell supernatanses showing quantity (pg) per mL tested sample
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Sandwich ELISA - PF4 (CXCL4) Mouse ELISA Kit (ab100735)PF4 measured in biofluids showing quantity (microgram) per mL of tested sample
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Typical standard curveRepresentative standard curve using ab100735
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Typical standard curveRepresentative standard curve using ab100735
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab100735 は 2 報の論文で使用されています。
- Xie Z et al. Monocyte upregulation of podoplanin during early sepsis induces complement inhibitor release to protect liver function. JCI Insight 5:N/A (2020). PubMed: 32641582
- Petzold T et al. ß1 integrin-mediated signals are required for platelet granule secretion and hemostasis in mouse. Blood 122:2723-31 (2013). Mouse . PubMed: 24004668