製品の概要

  • 製品名

    Mouse PAI1 Total ELISA Kit
    PAI1 キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    1 20 0.288ng/ml 0.023 7.9%
    2 20 1.64ng/ml 0.081 4.92%
    3 20 20.8ng/ml 1.86 8.95%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    1 10 0.307ng/ml 0.04 12.9%
    2 10 1.46ng/ml 0.155 10.6%
    3 10 16.6ng/ml 2.41 14.6%
  • サンプルの種類

    Serum, Plasma
  • アッセイタイプ

    Sandwich (quantitative)
  • 検出感度

    0.006 ng/ml
  • 検出範囲

    0.05 ng/ml - 50 ng/ml
  • 添加回収試験

    100.8 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Plasma 100.8 89.1% - 112%

  • 全工程の試験時間

    1h 50m
  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Mouse, Rabbit
  • 製品の概要

    Abcam’s Total PAI1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for is for the quantitative determination of active plasminogen activator inhibitor type 1 in mouse plasma. 

    Mouse PAI1 present in plasma reacts with the capture antibody coated and dried on a microtiter plate. Free, latent and complexed PAI1 will bind to the plate. Any unbound PAI1 is washed away and an anti-PAI1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the total PAI1 present in the samples, is then reacted with the secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of total PAI1 in the sample.

  • アプリケーション

    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Wash Buffer 1 x 50ml
    Anti-mouse PAI1 coated Microtiter Plate 1 unit
    Anti-mouse PAI1 Primary Antibody 1 vial
    Anti-rabbit horseradish peroxidase secondary antibody 1 x 10µl
    Mouse PAI1 Standard 1 vial
    TMB Substrate Solution 1 x 10ml
  • 研究分野

  • 機能

    Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.
  • 組織特異性

    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 関連疾患

    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 配列類似性

    Belongs to the serpin family.
  • 翻訳後修飾

    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 別名

    • Endothelial plasminogen activator inhibitor
    • PAI
    • PAI-1
    • PAI1
    • PAI1_HUMAN
    • PLANH1
    • Plasminogen activator inhibitor 1
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serpin E1
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • SERPINE1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab157529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • PAI1 measured in biological fluids showing quantity (ng) per mL of tested sample. Samples diluted 3-81 fold.

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参考文献

ab157529 has not yet been referenced specifically in any publications.

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