Mouse PAI1 ELISA Kit (SERPINE1) (ab197752)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 200 pg/ml
- Range: 298 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse PAI1 ELISA Kit (SERPINE1)
PAI1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 5 6.7% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 3 14.2% -
サンプルの種類
Cell culture supernatant, Serum, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
200 pg/ml -
検出範囲
298 pg/ml - 5000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 109 90% - 121% Cell culture media 83 72% - 88% Cit plasma 104 96% - 111% serum free media 117 104% - 128% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse -
製品の概要
Mouse PAI1 ELISA Kit (SERPINE1) (ab197752) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PAI1 (SERPINE1) protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PAI1 (SERPINE1) with 200 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Plasminogen activator inhibitor-1 (PAI1 or Serpin E1) is encoded by the Serpine1 gene. PAI1 is a serine protease inhibitor with a major role in the regulation of fibrinolysis. PAI1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), both of which are activators of plasminogen. PAI1 is a secreted protein produced by the endothelium as well as adipose tissue.
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試験プラットフォーム
Microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 1 x 384 tests 1 x 96 tests 10X Mouse PAI1 Capture Antibody 1 x 600µl 1 x 600µl 1 x 600µl 10X Mouse PAI1 Detector Antibody 1 x 600µl 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 1 unit 0 x 0 unit Antibody Diluent CPI2 1 x 6ml 1 x 6ml 1 x 6ml Mouse PAI1 Lyophilized Recombinant Protein (ab93068) 2 vials 2 vials 2 vials Plate Seals 1 unit 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 50ml 1 x 250ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 0 x 0 unit 1 unit Stop Solution 1 x 12ml 2 x 12ml 1 x 12ml TMB Development Solution 1 x 12ml 2 x 12ml 1 x 12ml -
研究分野
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機能
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. -
組織特異性
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells. -
関連疾患
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
配列類似性
Belongs to the serpin family. -
翻訳後修飾
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Clade E
- Endothelial plasminogen activator inhibitor
- Nexin
see all -
参照データベース
- Entrez Gene: 18787 Mouse
- SwissProt: P22777 Mouse
- Unigene: 250422 Mouse
関連製品
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Alternative Versions
画像
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Example of mouse PAI1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentration of native PAI1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of PAI1 were measured in duplicate and interpolated from the PAI1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1 concentration was determined to be 18.98 ng/mL in mouse serum and 7.69 ng/mL in mouse plasma (citrate).
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L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. PAI1 was measured in 10-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the PAI1 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 17.9 ng/mL unstimulated, 31.1 ng/mL stimulated, and undetectable in media.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab197752 は 6 報の論文で使用されています。
- Yuan C et al. Novel 1-hydroxy phenothiazinium-based derivative protects against bacterial sepsis by inhibiting AAK1-mediated LPS internalization and caspase-11 signaling. Cell Death Dis 13:722 (2022). PubMed: 35982051
- Nichols CE et al. Lrp1 Regulation of Pulmonary Function. Follow-Up of Human GWAS in Mice. Am J Respir Cell Mol Biol 64:368-378 (2021). PubMed: 33290178
- Ghosh AK et al. Pharmacological inhibition of PAI-1 alleviates cardiopulmonary pathologies induced by exposure to air pollutants PM2.5. Environ Pollut 287:117283 (2021). PubMed: 34426376
- Wiley CD et al. SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells. Cell Rep 28:3329-3337.e5 (2019). PubMed: 31553904
- Li B et al. MicroRNA-30b protects myocardial cell function in patients with acute myocardial ischemia by targeting plasminogen activator inhibitor-1. Exp Ther Med 15:5125-5132 (2018). PubMed: 29805539
- Ma J et al. Rivaroxaban attenuates thrombosis by targeting the NF-?B signaling pathway in a rat model of deep venous thrombus. Int J Mol Med 40:1869-1880 (2017). PubMed: 29039441