Mouse monoclonal [hlgA1] Anti-Human IgA H&L (ab433)

Submit a review Q&A (3)References (1)

Key features and details

  • Mouse monoclonal [hlgA1] Anti-Human IgA H&L
  • Host species: Mouse
  • Isotype: IgG1
  • Suitable for: ELISA, RIA

製品の概要

  • 製品名

    Mouse monoclonal [hlgA1] Anti-Human IgA H&L
    IgA 二次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [hlgA1] Anti-Human IgA H&L

  • 由来種

    Mouse

  • ターゲット生物種

    Human

  • 特異性

    hlgA1 specifically recognizes human IgA. hIgA - 100% hIgA (secretory) - 107% hIgA (myeloma) - 135% hIgA1 (myeloma) - 145% hIgA2 (myeloma) - 91% hIgA1 (myeloma) - 104% hIgA2 (myeloma) - 200% hIgA alpha-chain - 138% hIgG - <0.3% hIgG - <0.3% hIgM (plasma) - 2.6% hIgM (Waldenström's macroglobulinaemia) - <0.3% Affinity constant: 3x109 L/mol human plasma IgA.

  • アプリケーション

    適用あり: ELISA, RIAmore details

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 6.00
    Preservative: 0.095% Sodium azide
    Constituents: 0.335% PBS, 1.0878% Sodium citrate, 0.9% Sodium chloride
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    hlgA1

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    unknown

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab433の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.
特記事項
ELISA
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab433 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab433 は 1 報の論文で使用されています。

  • Juhela S  et al. T cell responses to enterovirus antigens and to beta-cell autoantigens in unaffected children positive for IDDM-associated autoantibodies. J Autoimmun 12:269-78 (1999). PubMed: 10330298

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Question

Is there anti human IgA2 conjugate [ Monoclonal or Polyclonal]other than ICN ?

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Answer

Thank you for your enquiry. Ab 433 is the only IgA secondary antibody that we have. To see if another company may have what you're looking for, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249).

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Question

Will this reagent cross react with monkey IgA? I need a single reagent that will detect human and monkey IgA, but not other classes of Ig.

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Answer

We have not tested the cross reactivity of this antibody (Mouse monoclonal to human IgA) with monkey IgA or with other monkey Ig classes. Its cross reativity with human IgG is below 0.3% and with human IgM(plasma) 2.6%. Human IgM(Waldenström's macroglobulinemia) cross reacts less than 0.3%.

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Question

Will this antibody recognize secreted IgA in the saliva? Does it crossreact with rat sIgA?

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Answer

Anti-IgA (ab433) cross-reacted 107% with the secretory IgA preparation from DAKO. Cross reacivity with rat and saliva as a sample have not yet been tested.

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