Mouse monoclonal [hIgG1] Anti-Human IgG H&L preadsorbed (ab436)

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Key features and details

  • Mouse monoclonal [hIgG1] Anti-Human IgG H&L preadsorbed
  • Host species: Mouse
  • Isotype: IgG2a
  • Suitable for: ELISA, RIA

製品の概要

  • 製品名

    Mouse monoclonal [hIgG1] Anti-Human IgG H&L preadsorbed
    IgG 二次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [hIgG1] Anti-Human IgG H&L preadsorbed

  • 由来種

    Mouse

  • ターゲット生物種

    Human

  • 特異性

    hIgG1 specifically recognizes human IgG. hIgG - 100% hIgG1 - 147% hIgG2 - 150% hIgG3 - 94% hIgG4 - 2.1% hIgM - 0.6% hIgA - 0.3% Goat IgG - <0.01% Rabbit IgG - <0.01% Horse IgG - <0.01% Pig IgG - <0.01% Cow IgG - <0.01% Sheep IgG - <0.01% Affinity Constant: 2x1010 L/mol human IgG.

  • アプリケーション

    適用あり: ELISA, RIAmore details

  • 吸着処理血清


    Cow, Goat, Horse, Pig, Rabbit, Sheep more details

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 6.00
    Preservative: 0.095% Sodium azide
    Constituents: 0.335% PBS, 1.0878% Sodium citrate, 0.9% Sodium chloride
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    hIgG1

  • アイソタイプ

    IgG2a

  • 軽鎖の種類

    unknown

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
RIA
  • 追加情報
    ELISA: Use at an assay dependant dilution.
    RIA: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab436 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab436 は論文での使用が確認できていません。

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    Question

    Hello,

    I am curious if any of the mouse anti-human IgG Mabs listed below cross react with monkey IgG?

    Mouse anti-Human IgG Fc secondary antibody (ab99757)

    Mouse monoclonal [H2] SecondaryAntibody to Human IgG - Fc (ab99770)

    Mouse anti-Human IgG1 Fc secondary antibody (ab1927)

    Mouse anti-Human IgG heavy chain secondary antibody (ab9243)

    Mouse monoclonal [hIgG1] SecondaryAntibody to Human IgG - H&L, pre-adsorbed (ab436)

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    Answer

    Thank you for contacting Abcam.

    We have not tested any of those antibodies against monkey IgG, therefore I cannot say whether they will cross react or not. Due to this, you can take advantage of our testing discount program if you want, please see the link below for more details:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



    If there is anything else I can help you with, please let me know.

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    Question

    I am hunting for an antibody that will distinguish between intact human IgG and single F(ab). Does this monoclonal Distinguish between intact IgG antibody and the F(ab).

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    Answer

    It is unknown as to which constant region of the heavy chain this antibody recognises, so we do not know if this antibody could distinguish between the two.

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    Question

    Please advise if this antibody has cross reactivity with chimpanzee or not. Yours sincerely, Yoko Kurozumo

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    Answer

    We have not tested the cross reactivity of this antibody with chimpanzee or with any other monkey IgA or with other monkey Ig classes. Its cross reativity with human IgG is below 0.3% and with human IgM(plasma) 2.6%. Human IgM(Waldenström's macroglobulinemia) cross reacts less than 0.3%.

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