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Mouse monoclonal [31-7-4] Anti-Human IgG2 Fc (HRP) (ab99779)

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ELISA - Mouse monoclonal [31-7-4] Anti-Human IgG2 Fc (HRP) (ab99779)

    Key features and details

    • Mouse monoclonal [31-7-4] Anti-Human IgG2 Fc (HRP)
    • Conjugation: HRP
    • Host species: Mouse
    • Isotype: IgG1
    • Suitable for: ELISA, Dot blot, IHC-Fr, ICC, WB

    Conjugates logo Related conjugates and formulations

    Unconjugated

    製品の概要

    • 製品名

      Mouse monoclonal [31-7-4] Anti-Human IgG2 Fc (HRP)
      IgG2 二次抗体 製品一覧

    • 由来種

      Mouse

    • ターゲット生物種

      Human

    • アプリケーション

      適用あり: ELISA, Dot blot, IHC-Fr, ICC, WBmore details

    • 免疫原

      Full length protein corresponding to IgG2. Human IgG2 myeloma protein

    • 標識

      HRP

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab99779の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ELISA (1)
    1/1000 - 1/2000.
    Dot blot
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    ICC
    Use at an assay dependent dilution.
    WB
    Use at an assay dependent dilution.
    特記事項
    ELISA
    1/1000 - 1/2000.
    Dot blot
    Use at an assay dependent dilution.
    IHC-Fr
    Use at an assay dependent dilution.
    ICC
    Use at an assay dependent dilution.
    WB
    Use at an assay dependent dilution.

    画像

    • ELISA - Mouse monoclonal [31-7-4] Anti-Human IgG2 Fc (HRP) (ab99779)
      ELISA - Mouse monoclonal [31-7-4] Anti-Human IgG2 Fc (HRP) (ab99779)

      ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted ab99779.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (6)

    ab99779 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab99779 は 6 報の論文で使用されています。

    • Chen Y  et al. A comprehensive, longitudinal analysis of humoral responses specific to four recombinant antigens of SARS-CoV-2 in severe and non-severe COVID-19 patients. PLoS Pathog 16:e1008796 (2020). PubMed: 32913364
    • Posadas-Mondragón A  et al. Association of Genetic Polymorphisms in TLR3, TLR4, TLR7, and TLR8 with the Clinical Forms of Dengue in Patients from Veracruz, Mexico. Viruses 12:N/A (2020). PubMed: 33138336
    • Henson D  et al. Association Between ApoA-I (Apolipoprotein A-I) Immune Complexes and Adverse Cardiovascular Events-Brief Report. Arterioscler Thromb Vasc Biol 39:1884-1892 (2019). PubMed: 31315438
    • Lenders M  et al. Characterization of drug-neutralizing antibodies in patients with Fabry disease during infusion. J Allergy Clin Immunol N/A:N/A (2018). PubMed: 29421273
    • Perugino CA  et al. Identification of galectin-3 as an autoantigen in patients with IgG4-related disease. J Allergy Clin Immunol N/A:N/A (2018). ELISA . PubMed: 29852256
    • Posadas-Mondragón A  et al. Indices of anti-dengue immunoglobulin G subclasses in adult Mexican patients with febrile and hemorrhagic dengue in the acute phase. Microbiol Immunol 61:433-441 (2017). PubMed: 28881485

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    ELISA-based IgG isotype identification in IgG-enriched samples from patients with Fabry disease

     Excellent
    Abreviews
    abreview image
    Application
    ELISA
    To control the general presence of IgG isotypes (IgG1, IgG2, IgG3, IgG4) and to check for IgEs and IgAs within purified total-IgG samples from serum ELISAs were performed as follows. Purified IgG fractions from 8 ERT-naive patient samples (patients 1 to 8) were pooled and 5.0 µg IgGs were immobilized on 96-well Maxisorp ELISA plates (Thermo Fisher Scientific) overnight at 48C, washed 3 times with PBS/Tween 0.1% and blocked with 100 µl PBS/BSA 2% for 2 hours at RT. Subsequently, 0.25 mg of IgG isotype-specific secondary antibodies (mouse a-human IgG isotype 1[ab99774] mouse a-human IgG isotype 2 [ab99779] rabbit a-human IgG isotype 3 [ab86253] mouse a-human IgG isotype 4 [ab99823] goat a-human IgA [ab97215] mouse a-human IgE [ab99806] Abcam, Cambridge, UK) were applied to the samples (in 100 µl PBS) and incubated for an additional 2 hours at RT. Rabbit a-human IgG isotype 3 was detected with goat a-rabbit IgG-HRP [sc-2054, Santa Cruz Biotechnology] as secondary antibody (diluted 1:5000). After 5 additional washes, 100 µl 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific) was added to each well, incubated for 10 minutes and stopped with 100 µl 2 mol/L sulfuric acid. Absorption was measured at 450 nm and calculated per microgram total IgG. Results demonstrated the measurable presence of IgG isotypes 1 to 4 within purified IgGs as well a moderate presence of IgAs of ERT-naive patients.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    投稿 Sep 12 2018

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