Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)

Abreviews (1)Q&A (5)Specific References (3)

製品の概要

  • 製品名
    Mouse monoclonal [2C11] Anti-Human IgG1 Fc
    IgG1 二次抗体 製品一覧
  • 由来種
    Mouse
  • ターゲット生物種
    Human
  • アプリケーション
    適用あり: IHC-Fr, ELISAmore details
  • 免疫原

    IgG from human serum.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項（精製）
    Chromatography on protein A Sepharose
  • ポリ/モノ
    モノクローナル
  • クローン名
    2C11
  • ミエローマ
    Sp2/0
  • アイソタイプ
    IgG1
  • 特記事項
    Concentration varies from lot to lot and can be provided on request.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration. PubMed: 24373342

参考文献

This product has been referenced in:
  • Girardi T  et al. The T-cell leukemia-associated ribosomal RPL10 R98S mutation enhances JAK-STAT signaling. Leukemia 32:809-819 (2018). Human . Read more (PubMed: 28744013) »
  • Marino N  et al. Development of a versatile oncolytic virus platform for local intra-tumoural expression of therapeutic transgenes. PLoS One 12:e0177810 (2017). Read more (PubMed: 28542292) »

See all 3 Publications for this product

ab1927 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

6 reviews or Q&A

10
Votes: Highest First

Mouse monoclonal 2C11 Anti-Human IgG1 Fc

 Excellent
Abreviews
Application
Flow Cytometry
Growing cells on plates coated with Fc-DII4, differentiating to proT cells through staining with CD44 and CD25.
Analyzed with FloCyt
We have been using this antibody for years and have used 5 different batches.
They have always done as promised in the datasheet.
abreview image
Username

Abcam user community

Verified customer

投稿 Apr 17 2017

I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
Does your company provid...

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Thank you for contacting us.

ab1927 does not cross react with mouse IgGs so it could also be a option to try.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.<...

Read More

I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
Does your company provid...

Read More

Thank you for contacting us.

Antibody ab50473 does not react with mouse IgGs. This might be suitable as per your requirements.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice...

Read More

I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
Does your company provid...

Read More

Thank you for contacting us.

It seems product ab1927 and ab50473, could be suitable however in order to confirm it I am now waiting, species cross reactivity data from lab. I will get back to you with more updates soon.

I hope this in...

Read More

Hello,

I am curious if any of the mouse anti-human IgG Mabs listed below cross react with monkey IgG?

Mouse anti-Human IgG Fc secondary antibody (ab99757)

Mouse monoclonal [H2] SecondaryAntibody to Human IgG - Fc (ab997...

Read More

Thank you for contacting Abcam.

We have not tested any of those antibodies against monkey IgG, therefore I cannot say whether they will cross react or not. Due to this, you can take advantage of our testing discount program if you want, pleas...

Read More

Is it IgG1, kappa or IgG1, lambda?

Ab1927 - (Monoclonal mouse anti-human IgG1) - recognizes both kappa and lambda allotypes of human IgG1. Itself is isotype IgG1, and we have not determined if it is kappa or lambda allotype.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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