Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)

Reviews (1)Q&A (5)References (5)

製品の概要

  • 製品名

    Mouse monoclonal [2C11] Anti-Human IgG1 Fc
    IgG1 二次抗体 製品一覧

  • 由来種

    Mouse

  • ターゲット生物種

    Human

  • アプリケーション

    適用あり: IHC-Fr, ELISAmore details

  • 免疫原

    IgG from human serum.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 特記事項（精製）

    Chromatography on protein A Sepharose

  • ポリ/モノ

    モノクローナル

  • クローン名

    2C11

  • ミエローマ

    Sp2/0

  • アイソタイプ

    IgG1

  • 特記事項

    Concentration varies from lot to lot and can be provided on request.

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration. PubMed: 24373342

画像

  • ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)
    ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)Marino et al PLoS One. 2017 May 18;12(5):e0177810. doi: 10.1371/journal.pone.0177810. eCollection 2017. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Characterisation of virus activity and functional expression of monoclonal antibodies.

    (Panel B) IgG1 antibody expression detected in cellular supernatants of HT-29 cells infected with 10ppc NG-135 by anti-IgG1 ELISA.

    Media was removed from infected or control cells, clarified by centrifugation and diluted 1 in 2 in 3% BSA/PBS for storage at -20°C. For quantification of human IgG1, 96 well plates (NUNC) were coated overnight, 4°C, with mouse monoclonal anti-human IgG1 Fc antibody (ab1927, Abcam) at a 1:1000 dilution in carbonate/bicarbonate buffer. Plates were blocked in 3% BSA/PBS, washed and samples and standards added to the plate for 1 hr and RT. Secondary detection was carried out using a HRP conjugated goat anti-human IgG Fab (ab87422, Abcam) incubated for 1 hr, RT.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献

ab1927 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1927 は 5 報の論文で使用されています。

  • Girardi T  et al. The T-cell leukemia-associated ribosomal RPL10 R98S mutation enhances JAK-STAT signaling. Leukemia 32:809-819 (2018). Human . PubMed: 28744013
  • Vanden Bempt M  et al. Cooperative Enhancer Activation by TLX1 and STAT5 Drives Development of NUP214-ABL1/TLX1-Positive T Cell Acute Lymphoblastic Leukemia. Cancer Cell 34:271-285.e7 (2018). PubMed: 30107177
  • Marino N  et al. Development of a versatile oncolytic virus platform for local intra-tumoural expression of therapeutic transgenes. PLoS One 12:e0177810 (2017). PubMed: 28542292
  • Medeiros MM  et al. Natural antibody response to Plasmodium falciparum merozoite antigens MSP5, MSP9 and EBA175 is associated to clinical protection in the Brazilian Amazon. BMC Infect Dis 13:608 (2013). ELISA ; Human . PubMed: 24373342
  • Hendaoui I  et al. Inhibition of Wnt/ß-catenin signaling by a soluble collagen-derived frizzled domain interacting with Wnt3a and the receptors frizzled 1 and 8. PLoS One 7:e30601 (2012). PubMed: 22303445

レビューと Q&A

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1-6 of 6 Abreviews or Q&A

Mouse monoclonal 2C11 Anti-Human IgG1 Fc

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Growing cells on plates coated with Fc-DII4, differentiating to proT cells through staining with CD44 and CD25.
Analyzed with FloCyt
We have been using this antibody for years and have used 5 different batches.
They have always done as promised in the datasheet.

Abcam user community

Verified customer

投稿 Apr 17 2017

Question

I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
Does your company provide such antibody. From the data sheet it is hard to judge because it always says that the antibody might cross react with other IgGs.
I would be very happy if you could get back to me.

Kind regards,

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Answer

Thank you for contacting us.

ab1927 does not cross react with mouse IgGs so it could also be a option to try.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
Does your company provide such antibody. From the data sheet it is hard to judge because it always says that the antibody might cross react with other IgGs.
I would be very happy if you could get back to me.

Kind regards,

Read More
Answer

Thank you for contacting us.

Antibody ab50473 does not react with mouse IgGs. This might be suitable as per your requirements.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
Does your company provide such antibody. From the data sheet it is hard to judge because it always says that the antibody might cross react with other IgGs.
I would be very happy if you could get back to me.

Kind regards,

Read More
Answer

Thank you for contacting us.

It seems product ab1927 and ab50473, could be suitable however in order to confirm it I am now waiting, species cross reactivity data from lab. I will get back to you with more updates soon.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

Hello,

I am curious if any of the mouse anti-human IgG Mabs listed below cross react with monkey IgG?

Mouse anti-Human IgG Fc secondary antibody (ab99757)

Mouse monoclonal [H2] SecondaryAntibody to Human IgG - Fc (ab99770)

Mouse anti-Human IgG1 Fc secondary antibody (ab1927)

Mouse anti-Human IgG heavy chain secondary antibody (ab9243)

Mouse monoclonal [hIgG1] SecondaryAntibody to Human IgG - H&L, pre-adsorbed (ab436)

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Answer

Thank you for contacting Abcam.

We have not tested any of those antibodies against monkey IgG, therefore I cannot say whether they will cross react or not. Due to this, you can take advantage of our testing discount program if you want, please see the link below for more details:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



If there is anything else I can help you with, please let me know.

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Question

Is it IgG1, kappa or IgG1, lambda?

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Answer

Ab1927 - (Monoclonal mouse anti-human IgG1) - recognizes both kappa and lambda allotypes of human IgG1. Itself is isotype IgG1, and we have not determined if it is kappa or lambda allotype.

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