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  1. Link

    mouse-monoclonal-2c11-human-igg1-fc-ab1927.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)

  • Datasheet
  • SDS
Reviews (1)Q&A (5)References (12)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)

    Key features and details

    • Mouse monoclonal [2C11] Anti-Human IgG1 Fc
    • Host species: Mouse
    • Isotype: IgG1
    • Suitable for: IHC-Fr, ELISA

    こちらの製品もご検討ください

    標識
    Product image
    Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)
    ブロッキング
    Normal Mouse Serum (sterile) (ab7486)

    関連製品

    製品の概要

    • 製品名

      Mouse monoclonal [2C11] Anti-Human IgG1 Fc
      IgG1 二次抗体 製品一覧
    • 由来種

      Mouse
    • ターゲット生物種

      Human
    • アプリケーション

      適用あり: IHC-Fr, ELISAmore details
    • 免疫原

      Full length native protein (purified) corresponding to Human IgG1.

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      pH: 7.40
      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 精製度

      Protein A purified
    • 特記事項(精製)

      Chromatography on protein A Sepharose
    • ポリ/モノ

      モノクローナル
    • クローン名

      2C11
    • ミエローマ

      Sp2/0
    • アイソタイプ

      IgG1
    • 特記事項

      Concentration varies from lot to lot and can be provided on request.
    • 研究分野

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Human
      • IgG
      • Unconjugated

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab1927の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration. PubMed: 24373342
    特記事項
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration. PubMed: 24373342

    画像

    • ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)
      ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (ab1927)Marino et al PLoS One. 2017 May 18;12(5):e0177810. doi: 10.1371/journal.pone.0177810. eCollection 2017. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Characterisation of virus activity and functional expression of monoclonal antibodies.

      (Panel B) IgG1 antibody expression detected in cellular supernatants of HT-29 cells infected with 10ppc NG-135 by anti-IgG1 ELISA.

      Media was removed from infected or control cells, clarified by centrifugation and diluted 1 in 2 in 3% BSA/PBS for storage at -20°C. For quantification of human IgG1, 96 well plates (NUNC) were coated overnight, 4°C, with mouse monoclonal anti-human IgG1 Fc antibody (ab1927, Abcam) at a 1:1000 dilution in carbonate/bicarbonate buffer. Plates were blocked in 3% BSA/PBS, washed and samples and standards added to the plate for 1 hr and RT. Secondary detection was carried out using a HRP conjugated goat anti-human IgG Fab (ab87422, Abcam) incubated for 1 hr, RT.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (12)

    ab1927 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1927 は 12 報の論文で使用されています。

    • Van Thillo Q  et al. Oncogenic cooperation between TCF7-SPI1 and NRAS(G12D) requires ß-catenin activity to drive T-cell acute lymphoblastic leukemia. Nat Commun 12:4164 (2021). PubMed: 34230493
    • Wang J  et al. Krüppel like factor 6 splice variant 1 (KLF6-SV1) overexpression recruits macrophages to participate in lung cancer metastasis by up-regulating TWIST1. Cancer Biol Ther 20:680-691 (2019). PubMed: 30590988
    • Zhao Z  et al. Upregulation of Insulin-Like Growth Factor-1 Receptor (IGF-1R) Reverses the Inhibitory Effect of Let-7g-5p on Migration and Invasion of Nasopharyngeal Carcinoma. Med Sci Monit 25:5747-5756 (2019). PubMed: 31374070
    • Wen Z  et al. Inhibition of lung cancer cells and Ras/Raf/MEK/ERK signal transduction by ectonucleoside triphosphate phosphohydrolase-7 (ENTPD7). Respir Res 20:194 (2019). PubMed: 31443651
    • Zhang Y  et al. Sirtuin 6 overexpression relieves sepsis-induced acute kidney injury by promoting autophagy. Cell Cycle 18:425-436 (2019). PubMed: 30700227
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Mouse monoclonal 2C11 Anti-Human IgG1 Fc

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Growing cells on plates coated with Fc-DII4, differentiating to proT cells through staining with CD44 and CD25.
    Analyzed with FloCyt
    We have been using this antibody for years and have used 5 different batches.
    They have always done as promised in the datasheet.

    Abcam user community

    Verified customer

    投稿 Apr 17 2017

    Question

    I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
    Does your company provide such antibody. From the data sheet it is hard to judge because it always says that the antibody might cross react with other IgGs.
    I would be very happy if you could get back to me.

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 11 2012

    Answer

    Thank you for contacting us.

    ab1927 does not cross react with mouse IgGs so it could also be a option to try.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jun 11 2012

    Question

    I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
    Does your company provide such antibody. From the data sheet it is hard to judge because it always says that the antibody might cross react with other IgGs.
    I would be very happy if you could get back to me.

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 08 2012

    Answer

    Thank you for contacting us.

    Antibody ab50473 does not react with mouse IgGs. This might be suitable as per your requirements.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jun 08 2012

    Question

    I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
    Does your company provide such antibody. From the data sheet it is hard to judge because it always says that the antibody might cross react with other IgGs.
    I would be very happy if you could get back to me.

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 08 2012

    Answer

    Thank you for contacting us.

    It seems product ab1927 and ab50473, could be suitable however in order to confirm it I am now waiting, species cross reactivity data from lab. I will get back to you with more updates soon.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jun 08 2012

    Question

    Hello,

    I am curious if any of the mouse anti-human IgG Mabs listed below cross react with monkey IgG?

    Mouse anti-Human IgG Fc secondary antibody (ab99757)

    Mouse monoclonal [H2] SecondaryAntibody to Human IgG - Fc (ab99770)

    Mouse anti-Human IgG1 Fc secondary antibody (ab1927)

    Mouse anti-Human IgG heavy chain secondary antibody (ab9243)

    Mouse monoclonal [hIgG1] SecondaryAntibody to Human IgG - H&L, pre-adsorbed (ab436)

    Read More

    Abcam community

    Verified customer

    Asked on Mar 19 2012

    Answer

    Thank you for contacting Abcam.

    We have not tested any of those antibodies against monkey IgG, therefore I cannot say whether they will cross react or not. Due to this, you can take advantage of our testing discount program if you want, please see the link below for more details:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



    If there is anything else I can help you with, please let me know.

    Read More

    Abcam Scientific Support

    Answered on Mar 19 2012

    Question

    Is it IgG1, kappa or IgG1, lambda?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 21 2005

    Answer

    Ab1927 - (Monoclonal mouse anti-human IgG1) - recognizes both kappa and lambda allotypes of human IgG1. Itself is isotype IgG1, and we have not determined if it is kappa or lambda allotype.

    Read More

    Abcam Scientific Support

    Answered on Apr 25 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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