Mouse MIP3 beta ELISA Kit (CCL19) (ab100729)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse MIP3 beta ELISA Kit (CCL19)
MIP-3 beta/CCL19 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 5 pg/ml -
検出範囲
4.1 pg/ml - 1000 pg/ml -
添加回収試験
100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 85.6 78% - 101% Serum 107.7 82% - 132% Plasma 119.5 83% - 140% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s MIP3 beta Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP-3- beta in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MIP3 beta coated on a 96- well plate. Standards and samples are pipetted into the wells and MIP3 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP3 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 260X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-Mouse MIP3 beta 2 vials MIP3 beta Microplate (12 x 8 wells) 1 unit Recombinant Mouse MIP3 beta Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. -
組織特異性
Expressed at high levels in the lymph nodes, thymus and appendix. Intermediate levels seen in colon and trachea, while low levels found in spleen, small intestine, lung, kidney and stomach. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Beta chemokine exodus 3
- Beta-chemokine exodus-3
- C C chemokine ligand 19
see all -
参照データベース
- Entrez Gene: 100039053 Mouse
- Entrez Gene: 24047 Mouse
- SwissProt: O70460 Mouse
- Unigene: 426373 Mouse
- Unigene: 441169 Mouse
- Unigene: 459263 Mouse
- Unigene: 6660 Mouse
画像
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Typical standard curve
Representative standard curve using ab100729
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab100729 は 2 報の論文で使用されています。
- Iida Y et al. Local injection of CCL19-expressing mesenchymal stem cells augments the therapeutic efficacy of anti-PD-L1 antibody by promoting infiltration of immune cells. J Immunother Cancer 8:N/A (2020). PubMed: 32675195
- Jenkins RW et al. Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids. Cancer Discov 8:196-215 (2018). PubMed: 29101162